The Effect of Soymilk on Alkaline Phosphatase, Total Antioxidant Levels, and Vasomotor Symptoms in Menopause Women

Document Type : Original Article

Authors

Biomedical Department, Women Research Center, Alzahra University, Tehran, Iran

Abstract

Objective
The aim of this study was to determine the effect of revival soymilk consumption and moderate exercise on vasomotor symptoms, total antioxidant capacity (TAC) and alkaline phosphates activity in postmenopausal women.
Materials and Methods
In this study, daily for 3 months, 37 postmenopausal women (mean age = 52.2± 4.6 years) were randomly assigned to soymilk (n=15), exercise +soymilk (n=12) and control (n= 10). The daily soymilk consisted of Revival brand Maxsoy, which contained 12.5 g of soy protein with Genistein (13 mg) and Daidzein (4.13 mg). Menopausal symptoms were measured using the Kupperman index, a standard measure of climacteric symptoms that has been validated in menopausal women at baseline and 3 months of daily consumption of soymilk. Total antioxidant status (TAS)of serum was measured using the ferric reducing ability of plasma (FRAP) assay. Total alkaline phosphatase (TALP) was assayed calorimetrically with Hitachi 902 autoanalyzer assay.
Results
Soymilk supplementation, soymilk and moderate exercise significantly (p<0.05) improved TAC level and TALP activity. Vasomotor symptoms were improved (p < 0.05) in the soymilk consumption plus moderate exercise and soymilk groups as compared with the control group. The data showed a decrease in menopausal symptoms p<0 .05, paired t test, two-tailed, between the baseline and soymilkgroups, and p < 0.05, ANOVA test, between the control  andtwo  groups. Vaginal problem, sexual symptoms and vasomotor significantly changed (p<0.05) after soymilk consumption.
Conclusion
These data may suggest that consumption of soymilk for 3 months enhances antioxidant and TALP status and reduces menopausal symptoms in postmenopausal women.

Keywords

References

1.Barrett CE. Epidemiology and the menopause: A global overview. Int J Fertil, 1993; 38:6-14.
2.Dempster DW, Lindsay R. Pathogenesis of osteoporosis. Lancet 1996; 341:797-801.
3.Andrews WC. The transitional years and beyond. Obstetrics & Gynecol 1995; 85(1):1-5.
4.Bonnier P, Romain S, Giacalone PL, Laffargue F, Martin PM, Piana L. Clinical and biologic prognostic factors in breast cancer diagnosed during postmenopausal hormone replacement therapy. Obstetrics & Gynecol 1995; 85(1):11-17.
5.Bolck G. A role of antioxidants in reducing cancer risks. Nutr Rev 1992; 50:207-213.
6.Boardley D, Fahlman M, Topp R, Morgan AL, McNevin N. The impact of exercise training on blood lipids in older adults. Am J Geriatr Cardiol 2007; 16:30-35.
7.Diplock A. Antioxidant nutrients- efficacy in disease prevention and safety. Biochemist 1995; 17:16-18.
8.Ji L. Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc 1993; 25: 225-231.
9.Nemoto K, Gen-no H, Masuki S, Okazaki K, Nose H. Effects of high-intensity interval walking training on physical fitness and blood pressure in middle-aged and older people. Mayo Clin Proc 2007; 82:803-811.
10.Kokkinos PF, Narayan P, Papademetriou V.Exerciseas hypertension therapy. Cardiol Clin 2001; 19:507-516.
11.Smith TC, Wingard DL, Smith B, Kritz-Silverstein D, Barrett-Connor E. Walking decreased risk of cardiovascular disease mortality in older adults with diabetes. J Clin Epidemiol 2007; 60:309-317.
12.Albright C, Thompson DL.  The effectiveness of walking in preventing cardiovascular disease in women: a review of the current literature. J Womens Health (Larchmt) 2006; 15:271-280.
13.Larun L, Nordheim LV, Ekeland E, Hagen KB, Heian F.  Exercise in prevention and treatment of anxiety and depression among children and young people. Cochrane Database Syst Rev 2006; 3, CD004691.
14.Elavsky S. Annals of Behavioral Medicine April 2007; online edition. Steriani Elavsky, PhD, assistant professor of kinesiology, Penn State University. Lila Nachtigall, MD, professor of ob-gyn, New York University School of Medicine, New York City.
15. Ross PD, Norimatsu H, Davis JW, Yano K, Wasnich RD. A comparison of hip frcture incidence amonge native Japanese, \ Japanese Americans and American Caucasians. Am J Epidemiol 1991; 133:801-809.
16.Albertazzi P, Pansini F, Bonaccorsi G. The effects of dietary soy supplementation on hot flushes. Obstet Gynecol 1998; 91: 6-11.
17.Simoon F. Food in china: a cultural and historical inquiry. Boca Raton, FL: CRC press Inc, 1991.
18.Kurzer MS, Xu X. Dietary phytoestrogens. Ann Rev Nutr, 1997; 17:353-381.
19.Coward L, Barnes NC, Setchell EDR, Barnes S. Genistein, daidzein and their glucoside conjugates: antitumor isoflavones in soybean foods from American diets and Asian diets. J Agri Food Chem 1993; 41:1961-1967.
20.Messina MJ. Legumes and soybeans. Overview of their nutritional profiles and health effects. Am J Clin Nutr 1999; 439-450.
21.Kuiper GG, Carisson B, Grandien K. Comparison of the ligand binding specifity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinol 1997; 138:863-870.
22.Roudsari AH, Tahbaz F, Hossein-Nezhad A, Arjmandi B, Larijani B, Kimiagar SM. Assessment of Soy Phytoestrogens' Effects on Bone Turnover Indicators in MenopausalWomen with Osteopenia in Iran: A Before and After Clinical Trial. Nutrition Journal  2005; 4:30 doi:10.1186/1475-2891-4-30
23.Dalais FS, Rice GE, Wahlqvist ML. Effects of dietary phytoestrogens in postmenopausal women. Climacteric 1998; 1: 124-129.
24.Adlercreutz H, Mazur W. Phytoestrogens and Western diseases. Ann Med 1997; 29:95-120.
25.Kupperman HS, Wetchler BB, Blatt MHG. Comtemporary therapy of the menopausal syndrome. JAMA 1959; 171:1627–37.
26.Benzie IFF, Strain JJ. Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996; 239:70-76.
27.Freedman RR. Physiology of hot flashes. Am J Human Biol 2001; 13:453- 64.
28.Evans E M, Racette S B. Effects of soy protein isolate and moderate exercise on bone turnover and bone mineral density in postmenopausal women. Menopause 2007; 14(3): 481-8.
29.Scambia G, Mango D, Signorile PG, Anselmi Angeli RA , Palena C, Gall Bombardelli E.  Clinical effects of a standardized soy extract in postmeonpausal women: A pilot study. Menopause 2000; 7:105–11.
30.Albertazzi P, Pansini F, Bonaccorsi G, Zanotti L, Forini E, De Aloysio D. The effect of dietary soy supplementation on hot flushes. Obstet Gynecol 1998; 91:6–11.
31. Murkies AL, Limbard C, Strauss BJ, Wilcox G, Burger HG, Morton MS. Dietary flour supplementation decreases post-menopausal hot flushes: Effect of soy and wheat. Maturitas 1995; 21:189–95.
32.Upmalis DH, Lobo R, Bradley L, Warren M, Cone FL, Lamia CA. Vasomotor symptom relief by soy isoflavone extract tablets in postmenopausal women: A multicenter, double-blind, randomized, placebo-controlled study. Menopause 2000; 7:236–42.
33.Glazier MG, Bowman MA. A review of the evidence for the use of phytoestrogens as a replacement for traditional estrogen replacement therapy. Arch Intern Med 2001; 161: 1161–72.
34. Crawford LE, Milliken EE, Irani K, Zweier JL, Becker LC, Johnson TM. Superoxide-mediated actin response in post-hypoxic endothelial cells. J Biol Chem 1996; 271: 26863–7.
35.Wei H, Bowen R, Cai Q, Barnes S, Wang Y. Antioxidant and antipromotional effects of the soybean isoflavone genistein. Proc Soc Exp Biol Med 1995; 208:124–30.
36.Potter SM, Baum JA, Teng H, Stillman RJ, et al. Soy protein and isoflavone: their effects on blood lipids and bone density in postmenopausal women. Am J Clin Nutr 1998; 68(suppl 6):1375-1379.
37. Alekel DL, Germain AS, Peterson CT, Hanson KB, et al. Isoflavonerich soy protein isolate attenuates bone loss in the lumbar spine of perimenopusal women. Am J Clin Nutr 2000; 72:844-852.
38.  Barnes S. Phytoestrogens and osteoporosis – What is a safe dose? Br J Nutr 2003; 39:101-108.

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