SCN1A and SCN1B gene polymorphisms and their association with plasma concentrations of carbamazepine and carbamazepine 10, 11 epoxide in Iranian epileptic patients

Document Type : Original Article

Authors

1 Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Pharmacotherapy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

3 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

4 Department of Medicinal Chemistry, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

5 Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

6 Department of Neurology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Objective (s): From a genetic point of view, epilepsy is a polygenic multifactorial syndrome. The SCN1A and B genes belong to a family of genes that provide instructions for making sodium channels. Understanding the relevance of SCN1A and SCN1B gene polymorphisms to plasma concentration of carbamazepine (CBZ) and its active metabolite carbamazepine 10, 11 epoxide (CBZE), may shed more light on inter-individual variations in response to CBZ.
Materials and Methods: In this cross-sectional study, genotype distribution and  allele frequency of six non-synonymous exonic single nucleotide polymorphisms (SNPs) of the SCN1A  and B genes were selected  and determined using PCR-RFLP in 70 epileptic patients treated with CBZ for at least 6 months. The patients had no hepatic or renal diseases and received no medications known to have a major interaction with CBZ. Serum concentrations of CBZ and CBZE were measured using High-Performance Liquid Chromatography (HPLC).
Results: The AA, AG and GG alleles of SCN1A were found in 23, 37 and 10 patients, respectively. There were no statistically significant differences in the mean (± standard deviation) of plasma concentrations of CBZ (P=0.8) and CBZE (P=0.1) among these 3 groups.  Likewise, there was no statistically significant relationship between SCN1A polymorphisms and CBZ concentration/dose ratio (P=0.7). A significant association was found between CBZ plasma level and CBZ concentration/dose with CBZ daily dose. All patients had the same genotype of SCN1B gene(CC). and no statistical analysis was performed.
Conclusion: No significant association between SCN1A gene polymorphisms and plasma levels of CBZ and CBZE were found.

Keywords

References

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Iranian Journal of Basic Medical Sciences
Volume 18, Issue 12 - Serial Number 12
December 2015
Pages 1215-1220

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