Protective effects of fractions from Artemisia biennis hydro-ethanolic extract against doxorubicin-induced oxidative stress and apoptosis in PC12 cells

Document Type : Original Article

Authors

1 Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Objective(s): This study was designed to indicate whether different fractions from Artemisia biennis hydroethanolic extract could provide cytoprotection against oxidative stress and apoptosis induced by doxorubicin (DOX) in rat pheochromocytoma cell line (PC12).
Material and Methods:Cell viability was determined by MTT assay. Also, activation of caspase-3 and superoxide dismutase were evaluated by spectrophotometry. Detection of reactive oxygen species (ROS) and measurement of mitochondrial membrane potential (MMP) were performed by flowcytometry.
Results:  Treatment of PC12 cells with DOX reduced viability dose dependently. For evaluation of the effect of fractions (A-G) on DOX-induced cytotoxicity, PC12 cells were pretreated for 24 hr with the A. biennis fractions and then cells were treated with DOX.  The fractions C and D increased PC12 cells viability significantly compared to DOX treated cells.  Moreover, pretreatment with fractions C and D for 24 hr attenuated DOX-mediated apoptosis and the anti-apoptotic action of A. biennis fractions was partially dependent on inhibition of caspase 3 activity and also increasing the  mitochondrial membrane potential (MMP). Selected A. biennis fractions also suppressed the generation of ROS and increased superoxide dismutase (SOD) activity.
Conclusion: Taken together our observation indicated that subtoxic concentration of aforementioned fractions of A. biennis hydroetanolic extract has protective effect against apoptosis induced by DOX in PC12 cell. The results highlighted that fractions C and D may exert cytoprotective effects through their antioxidant actions.

Keywords

References

1. Herrera B, Murillo MM, Alvarez-Barrientos A, Beltrn J, Fernndez M, Fabregat I. Source of early reactive oxygen species in the apoptosis induced by transforming growth factor-(beta) in fetal rat hepatocytes. Free Rad Biol Med 2004; 36:16-26.
2. Circu ML, Aw TY Reactive oxygen species, cellular redox systems, and apoptosis. Free Rad Biol Med 2008; 48:749-762.
3. Conklin KA. Chemotherapy-associated oxidative stress: impact on chemotherapeutic effectiveness. Integr Cancer Ther 2004; 3:294-300.
4. Hosseinzadeh L,  Behravan J, Mosaffa  F, Bahrami G, Bahrami A, Karimi G.  Curcumin potentiates doxorubicin-induced apoptosis in H9c2 cardiac muscle cells through generation of reactive oxygen species. Food Chem Toxicol 2011; 49:1102–1109.
5. Lopes AM, Meisel A, Dirnagl U, Carvalho FD, Bastos Mde L. Doxorubicin induces biphasic neurotoxicity to rat cortical neurons. Neurotoxicology 2008; 29:286-293.
6. Tangpogon J, Miriyala S, Noel T, Sinthupibulyakit C, Jungsuwadee P, St Clair DK. Doxorubicin induced central nevous system toxicity and protection by xanthone derivatives of Garcinia mangostana. Neuroscience 2011; 175:292–299.
7. Cardoso S, Santos  RX, Carvalho C, Correia S, Pereira G, Pereira S, Oliveira P, Santos MS, Proença T. Doxorubicin increases the susceptibility of brain mitochondria to Ca2+-induced permeability transition and oxidative damage. Free Radic  Biol Med 2008; 45:1395–1402.
8. Karlsson M, Kurz T, Brunk UT, Nilsson SE, Frennesson CI. “What does the commonly used DCF test for oxidative stress really show?” Biochem J 2010; 428:183–190.
9. Janelsins MC, Roscoe JA, Berg MJ, Thompson BD, Gallagher MJ, Morrow GR, et al. IGF-1 partially restores chemotherapy-induced reductions in neural cell proliferation in adult C57BL/6 mice. Cancer Invest 2010; 28:544–553.
10. Kesler s, Janelsins M, Koovakkattu D, Palesh O, Mustian K, Morrow G, et al. Reduced hippocampal volume and verbal memory performance associated with interleukin-6 and tumor necrosis factor-alpha levels in chemotherapy-treated breast cancer survivors. Brain Behav Immun 2013; 30:S109–S116.
11. Mozaffarian V. A Dictionary of Iranian Plant Names. Tehran: Farhang Moaser Publishers; 1978.
12. Nematollahi F, Rustaiyan A, Larijani K, Nadimi M. Essential oil composition of Artemisia biennis Willd and Pulicariaundulata (L.) C.A. Mey., two Compositae herbs growing wild in Iran. J Essent Oil Res 2006; 18:339–341.
13. Lopes-Lutz D, Alviano DS, Alviano CS, Kolodziejczyk PP. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 2008; 69:1732-1738.
14. Emami A, Zamani Taghizadeh Rabe SH, Ahi A, Mahmoudi M. Study on toxic effects of Artemisia spp. fractions from Iran on human cancer cell lines. J Zanjan Univ Med Sci 2010; 18:58-67.
15. Mojarrab M, Naderi R, Heshmati Afshar F. Screening of different extracts from Artemisia species for their potential antimalarial activity. Iran J Pharm Res 2015: 14:603-608.
16. Emami A, Zamani Taghizadeh Rabe SH, Ahi A, Mahmoudi M. Inhibitory activity of eleven Artemisia species from Iran against Leishmania major parasites. Iran J Basic Med Sci 2012; 15: 807-811.
17. Iranshahi M, Emami SA, Mahmoud-Soltani M. Detection of sesquiterpene lactones in ten Artemisia species population of Khorasan provinces. Iran J Basic Med Sci 2007; 10:183-188.
18. Singh KN, Lal B. Ethnomedicines used against four common ailments by the tribal communities of Lahaul-Spiti in Western Himalaya. J Ethnopharmacol 2008; 4; 115:147-159.
19. Hatami T, Emami SA, Miraghaee SS, Mojarrab M. Total phenolic contents and antioxidant activities of different extracts and fractions from the aerial parts of Artemisia biennis Willd. Iran J Pharm Res 2014; 13:551–559.
20. Hatanaka H. Nerve growth factor-mediated stimulation of tyrosine hydroxylase activity in a clonal rat pheochromocytoma cell line. Brain Res 1981; 222:225–233.
21. Rebois RV, Reynolds E, Toll L, Howard BD. Storage of dopamine and acetylcholine in granules of PC12, a clonal pheochromocytoma cell line. Biochemistry 1980; 19:1240–1248.
22. Klaassen CD. Casarett & Doull’s Toxicology. 7 th ed. McGraw Hill; 2007.p.30- 31.
23. Malstorm B, Andreasson L, Reinhammer B. The enzymeas. Boyer; 1975.p.533.
24. Hosseinzadeh L, Khorand A, Aliabadi A.  Discovery of 2- Phenyl –N-(5-(trifluoromethyl) – 1, 3, 4- thiadiazol-2-yl) acetamid derivatives as apoptosis inducer via caspase pathway whit potential anticancer activity. Arch Pharm Chem Life 2013; 346:812–818.
25. Shokoohinia Y, Hosseinzadeh L, Alipour M, Mostafaie A, Mohammadi-Motlagh HR. Comparative evaluation of cytotoxic and apoptogenic effects of several coumarins on human cancer cell lines: osthole induces apoptosis in p53-deficient H1299 cells. Adv Pharmacol Sci 2014; 2014:847574. 
26. Shokoohinia Y, Hosseinzadeh L, Moieni-Arya M, Mostafaie A, Mohammadi-Motlagh HR.  Osthole attenuates doxorubicin-Induced apoptosis in PC12 cells through inhibition of mitochondrial dysfunction and ROS production. Biomed Res Int 2014; 2014:156848.
27. Pal S, Ahir M, Sil PC. Doxorubicin-induced neurotoxicity is attenuated by a 43-kD protein from the leaves of Cajanus indicus L. via NF-κB and mitochondria dependent pathways. Free Radic Res 2012; 46:785-798.
28. Oz E, Ilhan. Effects of melatonin in reducing the toxic effects of doxorubicin. Mol Cell Biochem 2006; 286:11-15.
29. Zhang ZC, Su G, Li J, Wu H, Xie XD. Two new neuroprotective phenolic compounds from Gastrodiaelata. J Asian Nat Prod Res 2013; 15:619-623.
30. Choi RC, Zhu JT, Yung AW, Lee PS, Xu SL, Guo AJ, et al. Synergistic action of flavonoids, baicalein, and daidzein in estrogenic and neuroprotective effectspp A development of potential health products and therapeutic drugs against alzheimer's disease.  Evid Based Complementary Altern Med  2013; 63:56-94.
31. Shi J, Li CJ, Yang JZ, Yuan YH, Chen NH, Zhang DM. Coumarin glycosides and iridoid glucosides with neuroprotective effects from Hydrangea paniculata. Planta Med 2012; 78:1844-1850.
Iranian Journal of Basic Medical Sciences
Volume 19, Issue 5
May 2016
Pages 503-510

Files

Share

How to cite

Statistics