Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability

Document Type : Original Article

Authors

1 Anatomy and Cell Biology Department, Bushehr University of Medical Sciences and Health Services, Bushehr, Iran 2 Molecular and Cellular Biology Research Centre, Shaheed Beheshti University of Medical Sciences and Health Services, Tehran, Iran

2 Molecular and Cellular Biology Research Centre, Shaheed Beheshti University of Medical Sciences and Health Services, Tehran, Iran 3 Anatomy and Cell Biology Department, Yasuj University of Medical Sciences and Health Services, Yasuj, Iran

Keywords


Tucker M, Morton P, Liebermann J. Human oocyte cryopreserva
tion: a valid alternative to embryo cryopreservation?. Obstet Gynecol 2004; 113S:S24-S27.
2. Kim ChG, Yong H, Lee G, Cho J. Effect of the polyvinylpyrrolidone concentration of cryoprotectant on mouse embryo development and production of pups: 7.5% of PVP is beneficial for
 
 
In Vitro and In Vivo
development of frozen-thawed mouse embryos. J Reprod Dev 2008; 54:250-253.
3. Sheehan CB, Lane M, Gardner DK. The cryoloop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth. Hum Reprod 2006; 1:1-7.
 
4. Ciotti PM, Porcu E, Notarangelo L, Magrini O, Bazzocchi A, Venturoli S. Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing. Fertil Steril 2008; 91:2399- 2407.
 
5. Elder K, Dale B.
 
 
In vitro
fertilization. United Kingdom: Cambridge university press; 2003.
6. Makarevich AV, Chrenek P, Olexikova L, Popelkova M, Turanova Z, Ostro A,
 
 
et al
. Post-thaw survival, cell death and actin cytoskeleton in gene-microinjected rabbit embryos after vitrification. Theriogenology 2008; 70:675-681.
7. Almasi-turk S, Roozbehi A, Aliabadi E, Sadeghi Y, Haeri A, Hosseini A. Developmental consequences of mouse cryotop-vitrified oocyte and embryo using low concentrated CPAs. Iran J Reprod Med 2009; 7:181-188.
 
8. Eroglu A, Lawitts JA, Toner M, Toth TL. Quantitative microinjection of trehalose into mouse oocytes and zygotes, and its effect on development. Cryobiology 2003; 46:121-134.
 
9. Eroglu A, Bailey SE, Toner M, Toth TL. Successful cryopreservation of mouse oocytes by using low concentrations of trehalose and dimethylsulfoxide. Biol Reprod 2009; 80:70-78.
 
10. Kyono K, Nakajo Y, Kumagai S, Nishinaka C. Vitrifying and warming of oocytes using cryotop, In: Tucker MJ, Liebermann J, editors. Vitrification in assisted reproduction: A user’s manual and trouble shooting guide. United Kingdom: Informa healthcare; 2007. p. 153-161.
 
11. Soleimani R, Van der Elst J, Heytens E, Van den Broecke R, Gerris J, Dhont M,
 
 
et al.
Back muscle as a promising site for ovarian tissue transplantation, an animal model. Hum Reprod 2008; 23:619-626.
12. Balaban B, Urman B, Alatas C, Mercan R, Mumcu A, Isiklar A. A comparison of four different techniques of assisted hatching. Hum Reprod 2002; 17:1239-1243.
 
13. Zenzes MT, Bielecki R, casper RF, Leibo SP. Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes. Fertil Steril 2001; 75:769-777.
 
14. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: The Cryotop method. Theriogenology 2007; 67:73-80.
 
15. Gardner DK, Weissman A, Howless CM, Shoham Z.Text book of assisted reproductive techniques: Laboratory and clinical perspectives. United Kingdom: Taylor & Francis; 2004.
 
16. Tucker MJ, Liebermann J. Vitrification in assisted reproduction: A user’s manual and trouble shooting guide, United Kingdom: Informa healthcare; 2007.
 
17. Yamada C, Caetano HVA, Simoes R, Nicacio AC, Feitosa WB, Assumpcao MEOD. Improved bovine oocyte cryopreservation: Comparison of different associations with ethylene glycol, glycerol and dimethylsulfoxide. Anim Reprod Sci 2007; 99:384-388.
 
18. Endoh K, Mochida K, Ogonuki N, Ohkawa M, Shinmen A, Ito M,
 
 
et al.
The developmental ability of vitrified oocytes from different mouse strains assessed by parthenogenetic activation and intaracytoplasmic sperm injection. J Reprod Dev 2007; 53:1119-1206.
19. Kartberg AJ, Hambiliki F, Arvidsson T, Stravreus-Evers A, Svalander P. Vitrification with DMSO protects embryo membrane integrity better than solutions without DMSO. Reprod Biomed Online 2008; 17:378-384.
 
20. Manjunatha BM, Gupta PSP, Ravindra JP, Devaraj M, Nandi S. Effect of vitrification medium composition and exposure time on post-thaw development of buffalo embryos produced
 
 
in vitro.
Vet J 2009; 179: 287-291.
21. Cobo A, Kuwayamam M, Perezm S, Ruiz A, Pellicer A, Remohi J. Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the cryotop method. Fertil Steril 2008; 89:1657-1664.
 
22. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 2005; 11:300–308.
 
23. Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E. High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 2003; 80:223–224.
 
24. Morato R, Izquierdo D, Paramio MT, Mogas T. Cryotops versus open-pulled straws (OPS) as carriers for the cryopreservation of bovine oocytes: Effects on spindle and chromosome configuration and embryo development. Cryobiology 2008; 57:137-141.
 
25. Songsasen N, Yu IIJ, Ratterree MS, Vandevoort CA, Leibo SP. Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes. Fertil Steril 2002; 77:818-825.
 
26. Mandelbaum J, Anastasiou O, Levy R, Guerin JF, Larouziere V, Antoine JM. Effects of cryopreservation on the meiotic spindle of human oocytes. Obstet Gynecol 2004; 113S:S17-S23.
 
27. Nagai S, Mabuchi T, Hirata S, Shoda T, Kasai T, Yokota S. Correlation of abnormal mitochondria distribution in mouse oocytes with reduced developmental competence. Tohoku J Exp Med 2004; 210:137-144.
 
28. Bettencourt EMV, Bettencourt CM, Silva JNCE, Ferreira P, Matos CP, Oliveira E,
 
 
et al
. Ultrastructural characterization of fresh and cryopreservaed in vivo produced ovine embryos. Theriogenology 2009; 71:947-958.
29. Bromfield JJ, Coticchio G, Hutt K, Sciajno R, Borini A, Albertini DF. Meiotic spindle dynamics in human oocytes following slow-cooling cryopreservation. Hum Reprod 2009; 24:2114-2123.
 
30. Zhou GB, Li N. Cryopresrvation of porcine oocytes: recent advances. Mol Hum Reprod 2009; 15:279-285.
 
31. Mullen SF, Rosenbaum M, Critser JK. The effect of osmotic stress on the cell volume, metaphase II spindle and developmental potential of
 
 
in vitro
matured porcine oocytes. Cryobiology 2007; 54:281-289.
32. Shi WQ, Zhu SE, Zhang D, Wang WH, Tang GL, Hou YP
 
 
, et al
. Improved development by taxol pretreatment after vitrification of in vitro matured porcine oocytes. Reproduction 2006; 131:795-804.
33. Tharasanit T, Colenbrander B, Stout TAE. Effect of cryopreservation on the cellular integrity of equine embryos. Reprod Online 2005; 129:789-798.
 
34. Kim NH, Chung KS, Day N. The distribution and requirements of microtubules and microfilaments during fertilization and parthenogenesis in pig oocytes. J Reprod Fertil 1997; 111:143-149.
 
35. Paynter SJ. Current status of the cryopreservation of human unfertilized oocytes. Hum Reprod Update 2000; 6:449-456.
 
36. Isachenko EF, Nayudu PL. Vitrification of mouse germinal vesicle oocytes: effect of treatment temperature and egg yolk on chromatin and spindle normality and cumulus integrity. Hum Reprod 1997; 14:400-408.
 
37. Katayama M Zhongm Zh, Lai L, Sutovsky P, Prather RS, Schatten H. Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for develop
 
 
 
 
601
 
 
mental potential. Dev Biol 2006; 299:206-220.
38. Vajta G, Kuwayama M, Vanderzwalmen P. Disadvantages and benefits of vitrification, In: MJ Tucker, J Libermann J, editors. Vitrification in assisted reproduction. London: Informa Healthcare; 2007. p. 33-44.
39. Braga DPAF, Figueira RCS, Rodrigues D, Madaschi C, Pasqualotto FF, Iaconelli A, Borges E. Prognostic value of meiotic spindle imaging on fertilization rate and embryo development in
in vitro
-matured human oocytes. Fertil Steril 2008; 90:429-433.
40. Lowther KM, Weitzman VN, Maier D, Mehlmann LM. Maturation, fertilization, and the structure and function of the endoplasmic reticulum in cryopreserved mouse oocytes. Biol Reprod 2009; 81:147-154.
 
41. Gomes CM, Silva CASE, Acevedo N, Baracat E, Serafini P, Smith GD. Influence of vitirification on mouse metaphase II oocyte spindle dynamics and chromatin alignment. Fertil Steril 2008; 90:1396- 1404.
 
42. Amir A, Zvi R. Do chilling injury and heat stress share the same mechanism of injury in oocytes?. MCE 2008; 282:150-152.
 
43. Stachecki JJ, Cohen J, Schimmel T, Willadson SM. Fetal development of mouse oocytes and zygotes cryopreserved in a nonconventional freezing medium. Cryobiology 2002; 44:5-13.
 
44. Arav A, Yavin S. Measurement of essential physical properties of vitrification solutions. Theriogenology 2007; 67:81-89.
 
45. Pegg DE. Principles of Cryopreservation. Cryopreservation and freeze-drying protocols. In: JG Day, GN Stacey, editors. Methods in molecular biology. USA: Humana press Inc; 2006. p. 39-57.
 
46. Gardner DK, Sheehan CB, Rienzi L, Katz-jaffe M, Larman MG. Analysis of oocyte physiology to improve cryopreservation procedures. Theriogenology 2007; 67:64-72.
 
47. Wang WH, Meng L, Hackett RJ, Odenbourg R, Keefe DL. The spindle observation and its relationship with fertilization after intracytoplasmic sperm injection in living human oocytes. Fertil Steril 2001; 75:348-353.
 
48. Skidmore JA, Schovers E, Stout TAE. Effect of different methods of cryopreservation on the cytoskeletal integrity of dromedary camel (Camelus dromedaritus) embryos. Anim Reprod Sci 2008; 113:196-204.
 
49. Rienzi L, Martinez F, Ubaldi F, Minasi MG, Lacobelli M, tesarik J,
 
 
et al.
Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures. Hum Reprod 2004; 19:655-659.
50. Schroeder AC, Champlin AK, Mobraaten LE, Eppig JJ. Developmental capacity of mouse oocytes cryopreserved before and after maturation
 
 
in vitro.
J Reprod Fertil 1990; 89:43–50.
51. Zhang J, Nedambale TL, Yang M, Li J. Improved development of ovine matured oocyte following solid surface vitrification (SSV): Effect of cumulus cells and cytoskeleton stabilizer. Anim Reprod Sci 2009; 110:46-55.
 
52. Wang HY, Lu SS, Lun ZR. Glass transition behavior of the vitrification solutions containing propanediol, dimethyl sulfoxide and polyvinyl alcohol. Cryobiology 2009; 58:115-117.
 
53. Eroglu A, Toner M, Toth TL. Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes. Fertil Steril 2002; 77:152-158.
 
54. Stachecki JJ, Cohen J, Willadsoen S. Cryopreservation of unfertilized mouse oocytes: The effect of replacing sodium with choline in the freezing medium. Cryobiology 1998; 37:346-354.
 
55. Stachecki JJ, Cohen J, Willadsen S. Detrimental effects of sodium during mouse oocyte cryopreservation. Biol Rerod 1998; 59:395- 400.
 
56. Larman MK, Sheehan CB, Gardner DK. Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes. Reproduction 2006; 131:53-61.
 
57. Chian RC, Kuwayama M, Tan L, Tan J, Kato O, Nagai T. High survival rate of bovine oocytes matured in vitro following vitrification. J Reprod Dev 2004; 50:685-696.
 
58. Rupert-Lingham CJ, Paynter SJ, Godfrey J, Fuller BJ, Shaw RW. Developmental potential of murine germinal vesicle stage cumulus-oocyte complexes following exposure to dimethylsulphoxide or cryopreservation: Loss of membrane integrity of cumulus cells after thawing. Hum Reprod 2003; 18:392-398.
 
59. VandeVoort CA, Shirley CR, Hill DL, Leibo SP. Effects of CPAs and cryopreservation on germinal vesicle-stage cumulus-oocyte complexes of rhesus monkeys. Fertil Steril 2008; 90:805-816.