The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed rats

Document Type : Original Article

Authors

1 Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt

2 Department of Physiology, Faculty of Veterinary Medicine, Zagazig University

3 Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Ta’if, Saudi Arabia

4 Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt, 41522

Abstract

Objective(s): Hyperthermia is one of the most common environmental stressors that affect multi-biological systems in the body including the central nervous system as well as the hematopoietic organs. The objective of the present study was to investigate the protective role of ethanolic extract of propolis (EEP) on some selective stress markers, hematological, biochemical, and histopathological changes in rats subjected to hyperthermia (40 °C/12 hr).
Materials and Methods: The experimental groups (10 rats each) were classified as follows; Group A; control, (C), was kept at a controlled room temperature (25±5 °C). Group B; ethanolic extract of propolis, (EEP), was fed a basal diet supplemented with 3 g EEP/kg diet for 10 days. Group C; heat stress, (HS), was fed a basal diet for 10 days, and then exposed to high temperature (40±1 °C) for 12 hr. Group D; co-exposed, (EEP+HS) was fed a basal diet supplemented with 3 g EEP/kg diet for 10 days, and then subjected to high temperature (40±1 °C) for 12 hr. At the end of the experimental period, animals were decapitated; blood and tissue samples (brain and spleen) were collected for hematological, biochemical, and histopathological examination.
Results: EEP at a dose of 3 g/kg diet has a potent protective effect against hematotoxicity and brain damage as well as oxidative stress inducedby heat stress in rats.
Conclusion: The present study indicates that pre-treatment with EEP protects from hematotoxicity and neurological damage induced by high environmental temperature.

Keywords

References

1. Sharma HS. Hyperthermia induced brain edema: Current status & future perspectives. Indian J Med Res 2006; 123: 629-652.
2. Bouchama A, Knochel JP. Heat stroke. N Engl J Med 2002; 346:1978–1988.
3.  Patz JA, Campbell-Lendrum D, Holloway T, Foley JA. Impact of regional climate change on human health. Nature  2005; 438:310–317.
4. Imen BS, Taha N, Abdeljelil G, Hajer D, Moncef BM, Manef A. Reactive oxygen species, heat stress and oxidative-induced mitochondrial damage; A review. Int J Hyperthermia 2014; 30:513–523.
5. Chang CK, Chang CP, Liu SY, Lin MT. Oxidative stress and ischemic injuries in heat stroke. Prog Brain Res 2007; 162:525-546.
6. Brzezinska-Slebodzinska E. Species differences in the susceptibility of erythrocytes exposed to free radicals in vivo. Vet Res Commun 2003; 27:211–217.
7. Adenkola AY, Ayo JO. Effect of eight hours road transportation stress on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season. J  Cell Anim Biol 2009b; 3:004-008.
8. Amini-Sarteshnizi N, Mobini-Dehkordi M, Khosravi-Farsani S, Teimori H. Anticancer activity of ethanolic extract of propolis on AGS cell line. J HerbMed Pharmacol 2015; 4:29-34.
9. Jaqueline FC, Uilson PS, Paola SR, Marcio JD, José BPB, Claudia ALC, et al. Antimicrobial, antioxidant, anti-Inflammatory, and cytotoxic activities of propolis from the stingless Bee, Tetragonisca fiebrigi (Jataí). Evid Based Complement Alternat Med 2015; 1-11.
10. Séverine B, Anne-Marie LR,  Anne L,  Marie K, Viviane C, Catherine F, et al. Antifungal and antibacterial metabolites from a french poplar type propolis. Evid Based Complement Alternat Med 2015:1-10.
11. Guilherme RC,  Ronaldo ZM,  Karina  SV,  Cristina AF, Juliana CB,  Noemi T, et al. Antiviral action of hydromethanolic extract of geopropolis from Scaptotrigona postica against antiherpes simplex virus (HSV-1). Evid Based Complement Alternat Med 2015; 1-10.
12. Abeer ME, Amal AS, Faiza AM. The potential protective effect of propolis on experimentally induced hepatitis in adult male albino rats. Histological and immunohistochemical study. J Histol Histopathol 2015; 2:1-9.
13. Murat B, Sibel S, Mehtap Ö, Osman G, Nuri E, Mehmet B. In vivo nephroprotective efficacy of propolis against contrast-induced nephropathy. Diagn Interv Radiol  2015; 21: 317–321.
14. Thanaa AE, Ashraf ME, Nagla AE. Possible protective effect of propolis against lead induced neurotoxicity in animal model. J Evol Biol Res 2011; 3:4-11.
15. Enis YM, Yonar SM, Ural MŞ, Silici S, Düşükcan M. Protective role of propolis in chlorpyrifos-induced changes in the hematological parameters and the oxidative/antioxidative status of Cyprinus carpio carpio. Food Chem Toxicol 2012; 50:2703-2708.
16. Orun I, Selamoglu   Z, Gulhan MF, Erdogan K. Role of propolis on biochemical and hematological parameters of Oncorhynchus mykiss exposed to cypermethrin. J Survey Fisheries Sci 2014; 1:21-35.
17. Tatlı Seven P, Seval Y, Ismail S, Ibrahim HC, Mehmet AA, Mehmet Y. Effects of propolis on selected blood indicators and antioxidant enzyme activities in broilers under heat stress. Acta Vet Brno 2009; 78:75–83.
18. Joseph IM, Suthanthirarajan N, Namasivayam A. Effect of acute heat stress on certain immunological parameters in albino rat. Indian J Physiol Phannacol 1991; 35:269-271.
19. Feldman BR, Zinkl JG, Jain NC. Shalm’s veterinary hematology. 5th ed. Baltimore, Maryland: Lippincott, Williams & Wilkins; 2000.p.1344.
20. NCCLS. Methods for reticulocyte counting (Automated blood cell counters, flow cytometry, and supravital dyes); Approved guideline. 2nd ed. NCCLS, Wayne,Pennsylvania, USA: 2004.
21. Ruutu R. Determination of iron and unsaturated iron-binding capacity in serum with ferrozine. Clin  Chem  Acta  1975; 61:229 –232.
22. Torrance JD, Bothwell TH. Tissue iron stores. In: Methods in hematology: Iron (Cook, J D, ed) Churchill Livingstone, New York, NY: 1980.p.90-115.
23. Jendrassik G. Determination of serum bilirubin. Biochem1938; 297:81.
24. Vazquez-Palacios GP. Biochem Behavior 2001; 70:305-310.
25. Trinder P. Enzymatic method for glucose determination. Ann Clin  Biochem 1969; 6:24-28.
26. Hou C, Jia F, Liu, Y, Li L. CSF serotonin, 5-hydroxyindolacetic acid and neuropeptide Y levels in severe major depressive disorder. Brain Res 2006; 1095:154–158.
27. Ciarlone AE. Determination of catecholamines spectrophotofluro-metrically. Am J Physiol 1978; 125:731-737.
28. Seiler N. In methods of biochemical analysis (Glick, D, ed), 1970; 18:259-337.
29. Fernandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J Biol Chem 1994; 269:30761–30764.
30. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ.  Protein measurement with the folin phenol reagent. J Biol 1951; 193:265-275.
31. Ohkawa H, Ohishi N, Yagi K.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358.
32. Beutler E, Duron O, Kelly MB. Improved method for determination of blood glutathione. J Lab Clin Med 1963; 61: 882-888.
33. Beutler E. Reduced glutathione (GSH). In: Beutler E. editor. Red cell metabolism. A manual of biochemical methods. Grune and Straton, New York: 1975.
34. Bancroft JD, Gamble M. Theory and practice of histological techniques. 6th ed. London: Churchill Livingstone; 2008.
35. SPSS. Statistical package for social science, computer software, Ver 16. London, UK: SPSS Company; 2008.
36. Ahmed RG. Heat stress induced histopathology and pathophysiology of the central nervous system. Int J Dev Neurosci 2005; 23:549-557.
37. Larkindale J, Knight MR. Protection against heat stress-induced oxidative damage in arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol  2002; 128:682-695.
38. Klein JA, Ackerman SL. Oxidative stress, cell cycle, and neurodegeneration. J Clin Invest 2003; 111:785–793.
39. Mujahid A, Akiba Y, Toyomizu M. Acute heat stress induces oxidative stress and decreases adaptation in young white leghorn cockerels by downregulation of avian uncoupling protein. Poult Sci 2007; 86:364-371.
40. Triin K, Jaak K,  Vahur Ö,  Mihkel Z,  Kersti Z,  Jaan E, et al. Effects of heat acclimation on changes in oxidative stress and inflammation caused by endurance capacity test in the heat. Oxid Med Cell Longev 2014; 2014:1-8.
41. Boulant J. Mechanisms sensitivity to temperature. Ann N Y Acad Sci 2004; 856:108.
42. Faisal AB. Hazardous effects of hyperthermia on brain and testicular responses in rats. Life Sci J 2012; 9:992-996.
43. Beleen C, Martinez Fuentes AJ, Gracia Navarro F. Role of SST, COR and ghrelin and its receptors at the endocrine pancreas. Front Endocrinol 2012; 3:114.
44. Zhao Y, Xing C, Wang L. Effects of heat stress on DA mediated PI signal transduction system in rat straitum. Space Med Eng 2001; 14:116- 119.
45. Blatteis C, Li S, Li Z. Signaling the brain in systemic inflammation. The role of complement. Front Biosci 2004; 9:915.
46. Kinnersley AM, Turano FJ. Gamma aminobutyric acid (GABA) and plant responses to stress. Crit Rev Plant Sci 2000; 19:479–509.
47. Bouche N, Fromm H. GABA in plants: Just a metabolite? Trends Plant Sci 2004; 9:110–115.
48. Fesik SW, Shi Y. Structural biology; Controlling the caspases. Science 2001; 294:1477–1478.
49. Liu Y, Zheng Q, Wu H. The effects on expression ratio of Bax/Bcl-2 and the expression of activated caspase 3 in different types of tumor cells. Tumor 2013; 33:138–145.
50. Qian L, Xueli S, Huirong R, Jingbo G, Suqi C. Mitochondrial mechanism of heat stress-induced injury in rat cardiomyocyte. Cell Stress Chaperones  2004; 9:281–293.
51. Inoue H, Sameshima N, Ishida T, Tsuji A, Kudo K, Ikeda N. Vulnerability of experimentally induced fatty liver to heat stress in rats. J Gastroenterol  2006; 41:55-61.
52. Oladele  SB, Ayo JO, Ogundipe  SO, Esievo  KAN. Seasonal and species variations in erythrocytes osmotic fragility of indigenous poultry species in Zaria, Northern Guinea Savannah zone of Nigeria. Bull Anim Health Product Afr 2003; 51:204 -214.
53. Alhassan AW, Mohamed AM, Joseph OA, Ambali FS, Shittu M, Adenkola AY, Salawu EO. Effects of co-administration of antioxidants on erythrocyte osmotic fragility of Wistar rats during the hot-dry season. Eur J Sci Res 2010; 46:073-079.
54. Talas ZS, Dundar SP, Gulhan MF, Orun I,  Kakoolaki S. Effects of propolis on some blood parameters and enzymes in carp exposed to arsenic. Iran J Fish Sci 2012; 11:405–414.
55. Shapour K, Zeliha ST, Oguz C,  Osman C, Ilknur O. Role of propolis on oxidative stress in fish brain. Basic Clin Neurosci 2013; 4:153–158.
56. Kasai M, Fukumitsu H, Soumiya H, Furukawa S. Ethanol extract of chinese propolis facilitates functional recovery of locomotor activity after spinal cord injury. Evid Based Complement Alternat Med  2011; 2011.
Iranian Journal of Basic Medical Sciences
Volume 19, Issue 8
August 2016
Pages 875-882

Files

Share

How to cite

Statistics