Anti-hyperglycaemic and antioxidant effects of Bidens tripartita and quantitative analysis on its active principles

Document Type : Original Article

Authors

1 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Etiler, Ankara, Turkey

2 Republic of Turkey Social Security Institution, 06520, Balgat, Ankara, Turkey

3 Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06500, Tandogan, Ankara, Turkey

Abstract

Objective(s): Bidens species are used for their antidiabetic properties traditionally in many countries. Aim of this study is to evaluate hypoglycaemic and antidiabetic activity of Bidens tripartita extract and to identify its active compounds through bioactivity guided isolation technique.
Materials and Methods: Hypoglycaemic effects of B. tripartita extract and its sub-extracts were investigated in normal and glucose-hyperglycaemic rats. Streptozotocin induced diabetic rats were used to examine antidiabetic activity of the extract and its sub-extracts after acute and sub-acute administration. Additionally, in vitro enzyme inhibitory and antioxidant activities were evaluated. HPLC analyses were carried out to determine the active constituents of the extract and its sub-extracts.
Results: Through in vivo bioactivity-guided fractionation process, ethyl acetate and n-buthanol                  sub-extracts were found to have potent antidiabetic activity. In vitro enzyme inhibitory activities of the same sub-extracts were found to be potent. The highest total phenol, flavonoid contents and radical scavenging activity was determined in ethyl acetate sub-extract. According to LC-MS analyses, chlorogenic acid, luteolin and 7-O-glucoside of luteolin (cynaroside) were determined as the main components of the active sub-extracts.
Conclusion: According to our results, B. tripartita has potent antidiabetic activity and its active constituents might be beneficial for diabetes and its complications. 

Keywords

References

1.Sandu R, Miron A, Zagnat M, Ghiciuc C, Lupuşoru C. Experimental researches on acute toxicity of a Bidens tripartita extract in mice-preliminary investigation. Rev Med Chir Soc Med Nat Iasi 2012; 116: 1230-1234.
2.Kupicha K, Bidens L. In: Davis PH. editor. Flora of Turkey and the East Aegean Islands. 2th ed. E`dinburg: Edinburg University Press; Vol. 5. 1997. p. 46-47.
3.Sokolov SY. Phytotherapy and Phytopharmacology: The Manual for Doctors. Moscow: Medical News Agency; 2000.
4.Sezik E, Yesilada E, Shadidoyatov H, Kulivey Z, Nigmatullaev AM, Aripov HN, et al. Folk medicine in Uzbekistan I. Toshkent, Djizzax, and Samarqand provinces. J Ethnopharmacol 2004; 92: 197-207.
5.Blinova KF, Yakovlev GP. Botanical Pharmacognostic Dictionary. Moscow: Vysshayashkola; 1990.
6.Andrade-Cetto A, Heinrich M, Mexican plants with hypoglycaemic effect used in the treatment of diabetes. J Ethnopharmacol 2005; 99: 325-348.
7.Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine 1995; 2: 137-189.
8.Mootoosamy A, Mahomoodally MF. Ethnomedicinal application of native remedies used against diabetes and related complications in Mauritius. J Ethnopharmacol 2014; 151: 413-444.
9.Pereira RL, Ibrahim T, Lucchetti L, da Silva AJ, Goncalves de Moraes VL. Immunosuppressive and anti-inflammatory effects of methanolic extract and the polyacetylene isolated from Bidens pilosa L. Immunopharmacol 1999; 43: 31-37.
10.Tag H, Kalita P, Dwivedi P, Das AK, Namsa ND. Herbal medicines used in the treatment of diabetes mellitus in Arunachal Himalaya, northeast, India. J Ethnopharmacol 2012; 141: 787-795.
11.Ubillas RP, Mendez CD, Jolad SD, Luo J, King SR, Carlson TJ, et al. Antihyperglycemic acetylenic glucosides from Bidens pilosa. Planta Med 2000; 66: 82-83.
12.Morton JF. Spanish needles (Bidens pilosa L.) as a wild food resource. Econ Bot 1962; 16: 173-179.
13.Yang W-C. Botanical, pharmacological, phytochemical, and toxicological aspects of the antidiabetic plant Bidens pilosa LEvid Based Complement Alternat Med 2014; 2014:698617.
14.Shikov AN, Pozharitskaya ON, Makarov VG, Wagner H, Verpoorte R, Heinrich M. Medicinal plants of the Russian Pharmacopoeia; their history and applications. J Ethnopharmacol 2014; 154: 481-536.
15.International diabetes federation. key findings 2014. 2014. Available from: http://www.idf.org/diabetesatlas/update-2014 [Last access date: 20.11.2015]
16.Subramanian R, Asmawi MZ, Sadikun A. In vitro α-glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochim Pol 2008; 55: 391-398.
17.Alexiou P, Demopoulos VJ. Medicinal plants used for the treatment of diabetes and its long-term complications Plants, in: Kokkalou, E. (Ed.) Traditional and Modern Medicine: Chemistry and Activity. Kerala: Transworld Research Network; 2010. p. 69-175.
18.Ali H, Houghton PJ, Soumyanath A. α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006; 107: 449-455.
19.Lam SH, Chen JM, Kang CJ, Chen CH, Lee SS. α-Glucosidase inhibitors from the seeds of Syagrus romanzoffiana. Phytochemistry 2008; 69: 1173-1178.
20.Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex, specific application to the determination of vitamin E. Anal Biochem 1999; 269: 337-341.
21.Jung HA, Jin SE, Choi RJ, Manh HT, Kim YS, Min BS, et al. Anti tumorigenic activity of sophoraflavescenol against lewis lung carcinoma in vitro and in vivo, Arch Pharm Res 2011; 34: 2087-2099.
22.Zongo C, Savadogo A, Ouattara L, Bassole IHN, Ouattara CAT, Ouattara AS, et al. Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch. (Vitaceae): A medicinal plant from Burkina Faso. Int J Pharmacol 2010; 6: 880-887.
23.Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir-Knezevic S. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm 2004; 54: 65-72.
24.Alarcon-Aguilar FJ, Roman-Ramos R, Flores-Saenz JL, Aguirre-Garcia F. Investigation on the hypoglycaemic effects of extracts of four Mexican medicinal plants in normal and alloxan-diabetic mice. Phytother Res 2002; 16: 383-386.
25.Chang S-L, Chang CL-T, Chiang Y-M, Hsieh R-H, Tzeng C-R, Wu T-K, et al. Polyacetylenic compounds and butanol fraction from Bidens pilosa can modulate the differentiation of helper t cells and prevent autoimmune diabetes in non-obese diabetic mice. Planta Med 2004; 70: 1045-1051.
26.Chien S-C, Young PH,  Hsu Y-J, Chen C-H, Tien Y-J, Shiu S-Y, et al. Anti-diabetic properties of three common Bidens pilosa variants in Taiwan. J Ethnopharmacol 2009; 70: 1246-1254.
27.Dimo T, Rakotonirina SV, Tan PV, Azay J, Dongo E, Cros G. Leaf methanol extract of Bidens pilosa prevents and attenuates the hypertension induced by high-fructose diet in Wistar rats. J Ethnopharmacol 2002; 83: 183-191.
28.Hsu Y-J, Lee T-H, Chang CL-T, Huang Y-T, Yang W-C. Anti-hyperglycemic effects and mechanism of Bidens pilosa water extract. J Ethnopharmacol 2009; 122: 379-383.
29.Lv J-L, Zhang L-B. Flavonoids and polyacetylenes from the aerial parts of Bidens tripartita. Biochem Syst Ecol 2013; 48: 42-44.
30.Wolniak M, Tomczykowa M, Tomczyk M, Guedej J, Wawer I. Antioxidant activity of extracts and flavonoids from Bidens tripartita. Acta Pol Pharm 2007; 63: 441-447.
31.Andrade-Cetto A, Wiedenfeld H. Hypoglycemic effect of Cecropia obtusifolia on streptozotocin diabetic rats. Jl. Ethnopharmacol 2001; 78: 145-149.
32.Narita Y, Inouye K. Kinetic analysis and mechanism on the inhibition of chlorogenic acid and its components against porcine pancreas α-amylase isoenzymes I and II. J Agric Food Biochem 2009; 57: 9218-9225.
33.Oboh G, Agunloye OM, Adefegha SA, Akinyemi AJ, Ademiluyi AO. Caffeic and chlorogenic acids inhibit  key enzymes linked to type 2 diabetes (in vitro): a comparative study. J Basic and Clin Physiol Pharmacol 2015; 26: 165-170.
34.Tadera K, Minami Y, Takamatsu K, Matsuoka T. Inhibition of α-glucosidase and α-amylase by flavonoids. J Nutr Sci Vitaminol 2006; 52: 149-153.
35.Kato A, Minoshima Y, Yamamoto J, Adachi I, Watson AA, Nash RJ. Protective effects of dietary chamomile tea on diabetic complications. J Agric Food Chem 2008; 56: 8206-8211.
36.Kim J-S, Kwon C-S, Son KH. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Biosci Biotechnol Biochem 2000: 64; 2458-2461.
37.Rauter AP, Martins A, Borges C, Mota-Filipe H, Pinto R, Sepodes B, et al. Antihyperglycaemic and protective effects of flavonoids on streptozotocin-induced diabetic rats. Phytother Res 2010; 24: 133-138.
Iranian Journal of Basic Medical Sciences
Volume 19, Issue 10
October 2016
Pages 1114-1124

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