The Effects of a Moderate Exercise Program on Knee Osteoarthritis in Male Wistar Rats

Document Type : Original Article

Authors

1 Department of Sport Pathology and Corrective Exercises, School of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran

2 Department of Biology, Faculty of Sciences, University of Mazandaran, Babolsar, Iran

3 Department of Sport Pathology and Corrective Exercises, School of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

Abstract

 

Objective(s):
Osteoarthritis (OA) or degenerative joint disease is the commonest form of arthritis and can lead to joint pain, decrease in joint’s range of motion, loss of function, and ultimately disability. Exercise is considered as one of the non-pharmacological treatments of OA. But the effects of exercise on knee joint cartilage remain ambiguous. The aim of the present study was to investigate the effect of a four-week moderate treadmill exercise on rats’ knee osteoarthritis.
 
Materials and Methods:
Eighteen male Wistar rats (173 ± 1 g, 8 weeks old) were randomly divided into three groups (n = 6): Intact control, monosodium iodoacetate (MIA) only (OA), and training. The osteoarthritis model was induced by intra-articular injection of monosodium iodoacetate (MIA). Subjects followed a moderate-intensity exercise program for 28 days. Rats were killed after 28 days and histological assessment was done on their knee joints. One-way ANOVA (P<0.05) and post-hoc Tukey test was used for the statistical analysis.
Results:
Histological assessment on 3 measurements of, depth ratio of lesions (P=0.001), total cartilage degeneration width (P=0.001), and significant cartilage degeneration width (P=0.001), demonstrated that moderate exercise for 4 weeks could surprisingly almost treat OA symptoms of rats’ knee joints.
Conclusion:
The findings of the present study indicate that a moderate treadmill exercise program exert a beneficial influence on rats’ knee osteoarthritis.

Keywords

References

1. Jastan Marani M, Purfarzi F, Moharramzad Y. The effect of combination of glucosamine and chlorochin compared with glucosamine in the treatment of knee osteoarthritis. J Ardabil Univ Med Sci 2009; 9:157-163.
2. Nicholson S, Dickman K, Maradiegue A. Reducing premature osteoarthritis in the adolescent through appropriate screening. J Pediatr Nurs 2009; 24:69-74.
3. Rajaee A, Alirezaee A, Velaee N. The Comparison between effects of glucosamine and MSM in the treatment of knee osteoarthritis. J Shahid Beheshti Univ Med Sci 2009; 5:283-287.
4. Bitton R. The Economic Burden of Osteoarthritis. Am J Manag Care 2009; 15:230-235.
5. Gupta S, Hawker GA, Laporte A, Croxford R, Coyte PC. The economic burden of disabling hip and knee osteoarthritis (OA) from the perspective of individuals living with this condition. Rheumatology 2005; 44:1531–1537.
6. Bronner F, Farach-Carson M. Bone and Osteoarthritis. Springer 2007.
7. Darrow M. The Knee Sourcebook. 1st ed. McGraw-Hill; 2002.
8. Klug M, Shrier I, McBain K, Shultz R, Meeuwisse WH, Garza D, et al. The Prevention of Sport Injury: An Anlaysis of 12000 Published Manuscripts. Clin J Sport Med 2010; 20:407–412.
9. Hede A, Hejgaard N, Sandberg H, Jacobsen K. Sports injuries of the knee ligaments: a prospective stress radiographic study. Br J Sports Med 1985; 19:8-10.
10. Mikkelsson O, Nupponen H, Kaprio J, Kautiainen H, Mikkelsson M, Kujala M. Adolescent flexibility, endurance strength, and physical activity as predictors of adult tension neck, low back pain, and knee injury: a 25 year follow up study. Br J Sports Med 2006; 40:107–113.
11. Giza E, Mithöfer K, Farrell L, Zarins B, Gill T. Injuries in women's professional soccer. Br J Sports Med 2005; 39:212-216.
12. Hadipur M, Mozaffari R, Safavi M. The effect of pomegranate juice in protecting knee joint cartilage in experimental model of osteoarthritis. Med J Islam Azad Univ 2007; 17:199-203.
13. TM N, Heesch KC, Brown WJ. Efficacy of a progressive walking program and glucosamine sulphate supplementation on osteoarthritic symptoms of the hip and knee: a feasibility trial. Arthritis Res Ther 2010; 12:25-37.
14. Zhang W, Moskowitz RW. OARSI recommendations for the management of hip and knee osteoarthritis, Part II: OARSI evidence-based, expert consensus guidelines. Osteoarthritis Cartilage 2008; 16:137-162.
15. Clyman B. Exercise in the Treatment of Osteoarthritis. Current Rheumatol Reports 2001; 3:520–523.
16. Galois L, Etienne S, Grossin L, Watrin-Pinzano A, Cournil-Henrionnet C, Loeuille D, et al. Dose–response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study. OsteoArthritis Cartilage 2004; 12:779-786.
17. Cifuentes D, Rocha LG, Silva LA, Brito AC, Rueff-Barroso CR, Porto LC, et al. Decrease in oxidative stress and histological changes induced by physical exercise calibrated in rats with osteoarthritis induced by monosodium iodoacetate. Osteoarthritis Cartilage 2010; 18:1088-1095.
18. Saxon L, Finch C, Bass S. Sports participation, sports injuries and osteoarthritis: implications for prevention. Sports Med 1999; 28:123-135.
19. Lee YJ, Park JA, Yang SH, Kim KY, Kim BK, Lee EY, et- al. Evaluation of osteoarthritis induced by treadmill-running exercise using the modiWed Mankin and the new OARSI assessment system. Springer-Verlag 2010.
20. Kujala UM, Kaprio J, Sarna S. Osteoarthritis of weight bearing joints of lower limbs in former elite male athletes. Br Med J 1994; 308.
21. Blagojevic M, Jinks C, Jeffery A, Jordan KP. Risk factors for onset of osteoarthritis of the knee in older adults: a systematic review and meta-analysis. Osteoarthritis Cartilage 2010; 18:24-33.
22. Vignon E, Valat JP, Rossignol M, Avouac B, Rozenberg S, Thoumie P, et al. Osteoarthritis of the knee and hip and activity: a systematic international
Fallah Mohammadi M et al Exercise and Rat’s Knee Osteoarthritis Iran J Basic Med Sci, Vol. 16, No. 5, May 2013 688
review and synthesis (OASIS). Joint Bone Spine 2006; 73:442–455.
23. Sutton AJ, Muir KR, Mockett S, Fentem P. A case-control study to investigate the relation between low and moderate levels of physical activity and osteoarthritis of the knee using data collected as part of the Allied Dunbar National Fitness Survey. Ann Rheum Dis 2001; 60:756-64.
24. Kazeem A, Olubayo A, Ganiyu A. Plasma Nitric Oxide and Acute Phase Proteins after Moderate and Prolonged Exercises. Iran J Basic Med Sci 2012; 15:602-607.
25. Fehrenbacher A, Steck E, Rickert M, Roth W, Richter W. Rapid regulation of collagen but not metalloproteinase 1, 3, 13, 14 and tissue inhibitor of metalloproteinase 1, 2, 3 expression in response to mechanical loading of cartilage explants in vitro. Arch Biochem Biophys 2003; 410:39-47.
26. Mio K, Saito S, Tomatsu T, Toyama Y. Intermittent compressive strain may reduce aggrecanase expression in cartilage: a study of chondrocytes in agarose gel. Clin Orthop Relat Res 2005; 433:225-32.
27. Park S, Hung CT, Ateshian GA. Mechanical response
of bovine articular cartilage under dynamic unconfined compression loading at physiological stress levels. Osteoarthritis Cartilage 2004; 12:65-73.
28. Roos EM, Dahlberg Leif. Positive Effects of Moderate Exercise on Glycosaminoglycan Content in Knee Cartilage: A Four-Month, Randomized, Controlled Trial in Patients at Risk of Osteoarthritis. Arthritis Rheumatism 2005; 52:3507–3514.
29. Combe R, Bramwell S, Field MJ. The monosodium iodoacetate model of osteoarthritis: a model of chronic nociceptive pain in rats? Neurosci Lett 2004; 370:236–240.
30. Hadipur M, Mozaffari R. Protective effects of nutrients on articular cartilage of rats and its histological assessment. J Daneshvar Pezeshki 2008; 15:88-94.
31. Grewin N, Bendele M, Glasson S, Carlson S. The OARSI histopathology initiative- recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage 2010; 18:524-534.
32. Schuelert Niklas, McDougall Jason. Grading of monosodium iodoacetate-induced steoarthritis reveals a concentration-dependent sensitization of nociceptors in the knee joint of the rat. Neurosci Lett 2009; 465:184-188.
33. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-Iodoacetate-Induced Histologic Changes in Subchondral Bone and Articular Cartilage of Rat Femorotibial Joints: An Animal Model of Osteoarthritis. Toxicol Pathol 2003; 31:619-624.
34. Roman-Blas JA, Jimenez SA. NF-kB as a potential therapeutic target in osteoarthritis and rheumatoid arthritis: Review. Osteoarthritis Cartilage 2006; 14:839-848.
35. Lay E, Samiric T, Handley CJ, Ilic MZ. Short- and long-term exposure of articular cartilage to curcumin or quercetin inhibits aggrecan loss. J Nutr Biochem 2011.
36. Ghosh P. The pathobiology of osteoarthritis and the rationale for the use of pentosan polysulfate for its treatment. Semin Arthritis Rheum 1999; 28:211–67.
37. Reginster JY, Deroisy R, Rovati LC, Lee RL, Lejeune E, Bruyere O, et al. Long-term effects of glucosamine sulphate on osteoarthritis progression: a randomised, placebo-controlled clinical trial. Lancet 2001; 357:251–256.
38. Knobloch TJ, Madhavan S, Nam J, Agarwal Jr S, Agarwal S. Regulation of chondrocytic gene expression by biomechanical signals. Crit Rev Eukaryot Gene Expr 2008; 18:139-150.
39. Pinho RA, Andrades ME, Oliveira MR, Pirola AC, Zago MS, Silveira PCL, et al. Imbalance in SOD/CAT activities in rat skeletal muscles submitted to treadmill training exercise. Cell Biol Int 2006; 30:848-853.
40. Meydani M, Evans W, Handelman G, Fielding RA, Meydani SN, Fiatarone MA, et al. Antioxidant response to exercise-induced oxidative stress and protection by vitamin E. Ann N Y Acad Sci 1992; 669:363-374.
41. Powers SK, Criswell D, Lawler J, Ji LL, Martin D, Herb RA, et al
. Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle. Am J Physiol 1994; 266:375-380.

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