Effect of sodium hydrosulfide on mRNA expression of prostaglandin E2 receptors in response to mucosal acidification and distention-induced gastric acid secretion in rats

Document Type: Original Article

Authors

1 Physiology Research Center and Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Physiology Research Center and Department. of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Objective(s): Prostaglandins have been shown to mediate the gastro-protective effect of sodium hydrosulfide (NaHS) but effect of NaHS on mRNA expression of prostaglandin E2 receptors (EP1, 3-4; EPs) has not been investigated. Therefore, this study designed to evaluate the effect of NaHS on mRNA expression of EPs receptors in response to mucosal acidification and distention-induced gastric acid secretion in rats.
Materials and Methods: Fasted rats were randomly assigned into 4 groups (n=6/group). They were control, and NaHS-treated groups. To evaluate the effect of NaHS on mucosal mRNA expression of EPs receptors, the gastric mucosa exposed to stimulated gastric acid output and mucosal acidification. The pylorus sphincter catheterized for instillation of isotonic neutral saline or acidic solution. Ninety min after beginning the experiments, animals sacrificed and the gastric mucosa collected to determine the pH, mucus secretion and to quantify the mRNA expression of EPs receptors by quantitative real-time PCR.
Results: present results showed that a) NaHS increased the mucus secretion, mRNA expression of EP3 and EP4 receptors in response to distention-induced expression; b) The mRNA expression of EP1 receptors increased while EP4 mRNA receptors decreased in response to mucosal acidification in NaHS-pretreated rats; and c) NaHS increased pH of gastric contents both in response to distention-induced gastric acid secretion and mucosal acidification.
Conclusion: NaHS behaves in a different manner. It effectively only increased the pH of gastric contents to reinforce the gastric mucosa against a highly acidic solution but modulated both acid and mucus secretion when the rate of acid increase in the stomach was slower.

Keywords


1. Araki H, Ukawa H, Sugawa Y, Yagi K, Suzuki K, Takeuchi K. The roles of prostaglandin E receptor subtypes in the cytoprotective action of prostaglandin E2 in rat stomach. Aliment Pharmacol Ther 2000; 14:116-124.
2. Ding M, Kinoshita Y, Kishi K, Nakata H, Hassan S, Kawanami C, et al. Distribution of prostaglandin E receptors in the rat gastrointestinal tract. Prostaglandins 1997; 53:199-216.
3. Dey I, Lejeune M, Chadee K. Prostaglandin E2 receptor distribution and function in the gastrointestinal tract. Br J Pharmacol 2006; 149:611-623.
4. Kato S, Aihara E, Yoshii K, Takeuchi K. Dual action of prostaglandin E2 on gastric acid secretion through different EP-receptor subtypes in the rat. Am J Physiol Gastrointest Liver Physiol 2005; 289:G64-69.
5. Takeuchi K, Aihara E, Hayashi M, Sasaki Y. Role of prostaglandin E receptor subtypes in gastroduodenal HCO3- secretion. Med Chem 2005; 1:395-403.
6. Wallace JL. Hydrogen sulfide: a rescue molecule for mucosal defence and repair. Dig Dis Sci 2012; 57:1432-1434.
7. Mard SA, Askari H, Neisi N, Veisi A. Antisecretory effect of hydrogen sulfide on gastric acid secretion and the involvement of nitric oxide. Bio Med Res Int 2014; 2014:480921.
8. Takeuchi K, Ise F, Takahashi K, Aihara E, Hayashi S. H2S-induced HCO3- secretion in the rat stomach--involvement of nitric oxide, prostaglandins, and capsaicin-sensitive sensory neurons. Nitric Oxide 2015; 46:157-164
9. Magierowski M, Jasnos K, Kwiecien S, Drozdowicz D, Surmiak M, Strzalka M, et al. Endogenous prostaglandins and afferent sensory nerves in gastroprotective effect of hydrogen sulfide against stress-induced gastric lesions. PloS One 2015; 10:e0118972.
10. Cheraghi P, Mard SA, Nagi T. Hydrogen sulfide upregulated mRNA expressions of Sodium bicarbonate cotransporter1, Trefoil factor1 and Trefoil factor2 in gastric mucosa in rats.Vet Res Forum. accepted to be published.
11. Mard SA, Veisi A, Ahangarpour A, Gharib-Naseri MK. Gastric acid induces mucosal H2S release in rats by upregulating mRNA and protein expression of cystathionine gamma lyase.  J Physiol Sci 2015; 65:545-554.
12. Mard SA, Godarzinejad H, Dianat M. Duodenal acidification stimulates gastric H2S release through upregulating mRNA expression of cystathionine gamma lyase. Physiol Pharmacol 2016; 20:57-62.
13. Northey A, Denis D, Cirino M, Metters KM, Nantel F. Cellular distribution of prostanoid EP receptors mRNA in the rat gastrointestinal tract. Prostaglandins  Other Lipid Mediat 2000; 62:145-156.