Protective effect of Crocus sativus L. (Saffron) extract on spinal cord ischemia-reperfusion injury in rats

Authors

1 Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2 Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

3 Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Abstract

Objective(s): Ischemia/reperfusion (I/R) injury of spinal cord is leading to the paraplegia observed. In this study, we investigated the protective effect of the saffron extract on spinal cord I/R injury.
Materials and Methods: Thirty five male Sprague-Dawley rats were divided into 5 groups: intact, sham surgery, normal saline (NS), low dose saffron aqua extract, high dose saffron aqua extract.
Results: The mean motor deficit index (MDI) scores were significantly lower in the saffron extract groups than in the NS group at 48 hr after spinal cord ischemia (P<0.001). Saffron extract groups significantly decreased plasma level of malondialdehyde than in the NS Group (P<0.05). The number of motor normal neurons was significantly greater in the high saffron extract group than in the NS and low saffron group (P<0.05).
Conclusion: These data suggest that a saffron extract may protect spinal cord neurons from I/R injury.

Keywords

References

1. Eltzschig HK, Collard CD. Vascular ischemia and reperfusion injury. Br Med Bull 2004; 70:71-86.
2. Li XQ, Lv HW, Tan WF, Fang B, Wang H, Ma H. Role of the TLR4 pathway in blood-spinal cord barrier dysfunction during the biomodal stage after ischemia/reperfusion injury in rats. J Neuroinflamm 2014; 11:62.
3. Wan IY, Angelini GD, Bryan AJ, Ryder I, Underwood MJ. Prevention of spinal cord ischemia during descending thoracic and thoracoabdominal aortic surgery. Eur J Cardiothorac Surg 2001; 19:203-213.
4. Coyle JT, Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science 1993; 262:689-695.
5. Saito T, Tsuchida M, Umehara S, Kohno T, Yamamoto H, Hayashi J. Reduction of spinal cord ischemia/reperfusion injury with simvastatin in rats. Anesth Analg 2011; 113:565-567.
6. Tamaddonfard E, Farshid AA, Eghdami K, Samadi F, Erfanparast E. Comparison of the effects of crocin, safranal and diclofenac on local inflammation and inflammatory pain responces induced by carrageenan in rats. Pharmacol Rep 2013; 65:1272-1280.
7. Boskababy MH, Tabatabaee A, Byrami G. The effect of the extract of Crocus sativus and its constituent safranal, on lung inflammation of ovalbumin sensitized guinea-pigs. Phytomedicine 2012; 19:904-911.
8. Hossenzadeh H, Sadeghnia HR. Safranal, a constituent of Crocus sativus (saffron), attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sci 2005; 8:394-399.
9. Hossenzadeh H, Modaghegh MH, Saffari Z. Crocus sativus L. (saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle. Evid Based Complement Alternat Med 2009; 6:343-350.
10. Makhlouf H, Saksouk M, Habib J, Chahine R. Determination of antioxidant activity of saffron taken from the flower of Crocus sativus grown in Lebanon. Afr J Biotechnol 2011; 10:8093-8100.
11. Wang B, Zhu Q, Man X, Guo L, Hao L. Ginsenoside Rd inhibits apoptosis following spinal cord ischemia/reperfusion injury. Neural Regen Res 2014; 9:1678-1687.
12. Mehdizadeh R, Parizadeh MR, Khooei AR, Mehri S, Hosseinzadeh H. Cardioprotective effect of saffron
extract and safranal in isoproterenol-induced myocardial infarction in Wister rats. Iran J Basic Med Sci 2013; 16:56-63.
13. Taira Y, Marsala M. Effect of proximal arterial perfusion pressure on function, spinal cord blood flow, and histopathologic changes after increasing intervals of aortic occlusion in the rat. Stroke 1996; 27:1850-1858.
14. Asdag SM, Inamdar MN. Potential of Crocus sativus (saffron) and its constituent, crocin, as hypolipidemic and antioxidant in rats. Appl Biochem Biotechnol 2010; 162:358-372.
15. Akuzawa S, Kazui T, Shi E, Yamashita K, Bashar AH, Terada H. Interleukin-1 receptor antagonist attenuates the severity of spinal cord ischemia injury in rabbits. J Vasc Surg 2008; 48:694-700.
16. Hossenzadeh H, Khosravan V. Anticonvulsant effects of aqueous and ethanolic extracts of Crocus sativus L. stigmas in mice. Arch Iran Med 2002; 5:44-47.
17. Nam KN, Park YM, Jung HJ, Lee JY, Min BD, Park SU, et al. Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur Pharmacol 2010; 648:110-116.
18. Bulger EM, Maier RV. Antioxidants in critical illness. Arch Surg 2001; 136:1201-1207.
Iranian Journal of Basic Medical Sciences
Volume 20, Issue 3
March 2017
Pages 334-337

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