Melatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos

Document Type : Original Article

Authors

1 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Anatomical Science Research Center, Kashan University of Medical Sciences, Kashan, Iran

3 Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

4 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iranv

Abstract

Objective(s): To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4).
Materials and Methods: Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10-9 M melatonin) and Luzindole-treated (10-9 M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the level of intracellular ROS, antioxidant capacity, and also the number of cells were evaluated and compared with the fourth group in vivo developed blastocysts (control group).
Results: We concluded that melatonin significantly up-regulated the ErbB1 and ErbB4 gene and protein expression, decreased intracellular ROS, increased the total antioxidant capacity, and also elevated the cell numbers in the melatonin-treated group compared with the other groups (P≤0.05).
Conclusion: The use of melatonin may be a helpful factor in improving the embryo quality and enhancing the expression of ErbB1 and ErbB4, two important implantation-related genes and proteins.

Keywords

References

1. Peters VM, Spray DC, Mendez-Otero R. Effect of mesenchymal stem cells and mouse embryonic fibroblasts on the development of preimplantation mouse embryos. In Vitro Cell Dev Bidl Anim 2016; 52:497-506.
2. Yu S, Long H, Lyu Qf, Zhang Qh, Yan Z-g, Liang Hx, et al. Protective effect of quercetin on the development of preimplantation mouse embryos against hydrogen peroxide-induced oxidative injury. PLoS One 2014; 9:e89520.
3. Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N. Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta 2013; 1833:3448-3459.
4. Mehaisen GM, Saeed AM, Gad A, Abass AO, Arafa M, El-Sayed A. Antioxidant capacity of melatonin on preimplantation development of fresh and vitrified rabbit embryos: morphological and molecular aspects. PLoS One 2015; 10:e0139814.
5. Wrenzycki C, Herrmann D, Keskintepe L, Martins A Jr, Sirisathien S, Brackett B, et al. Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos. Hum Reprod 2001; 16:893-901.
6. Jeong W, Jung S, Bazer FW, Song G, Kim J. Epidermal growth factor: Porcine uterine luminal epithelial cell migratory signal during the peri-implantation period of pregnancy. Mol Cell Endocrinol 2016; 420:66-74.
7. Lim HJ, Dey S. HB-EGF: a unique mediator of embryo-uterine interactions during implantation. Exp Cell Res 2009; 315:619-626.
8. Gao C, Han HB, Tian XZ, Tan DX, Wang L, Zhou GB, et al. Melatonin promotes embryonic development and reduces reactive oxygen species in vitrified mouse 2-cell embryos. J Pineal Res 2012; 52:305-311.
9. Landis G, Shen J, Tower J. Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster. Aging 2012; 4:768-789.
10. Movaghar B, Askarian S. Expression of e-cadherin, leukemia inhibitory factor and progesterone receptor in mouse blastocysts after ovarian stimulation. Cell J 2012; 14:225-230.
11. Moshkdanian G, Nematollahi-mahani SN, Pouya F, Nematollahi-mahani A. Antioxidants rescue stressed embryos at a rate comparable with co-culturing of embryos with human umbilical cord mesenchymal cells. J Assist Reprod Genet 2011; 28:343-349.
12. Brown N, Deb K, Paria BC, Das SK, Reese J. Embryo-uterine interactions via the neuregulin family of growth factors during implantation in the mouse. Biol Reprod 2004; 71:2003-2011.
13. Parks JC, McCallie BR, Janesch AM, Schoolcraft WB, Katz-Jaffe MG. Blastocyst gene expression correlates with implantation potential. Fertil Steril 2011; 95:1367-1372.
14. Wang H, Dey SK. Roadmap to embryo implantation: clues from mouse models. Nat Rev Genet 2006; 7:185-199.
15. Altmäe S, Reimand J, Hovatta O, Zhang P, Kere J, Laisk T, et al. Research resource: interactome of human embryo implantation: identification of gene expression pathways, regulation, and integrated regulatory networks. Mol Endocrinol 2011; 26:203-217.
16. Gupta S, Malhotra N, Sharma D, Chandra A, Ashok A. Oxidative stress and its role in female infertility and assisted reproduction: clinical implications. Int J Fertil Steril 2009; 2:147-164.
17. Agarwal A, Durairajanayagam D, du Plessis SS. Utility of antioxidants during assisted reproductive techniques: an evidence based review. Reprod Biol Endocrinol 2014; 12:112.
18. Nematollahi-mahani SN, Nematollahi-mahani A, Moshkdanian G, Shahidzadehyazdi Z, Labibi F. The role of co-culture systems on developmental competence of preimplantation mouse embryos against pH fluctuations. J Assist Reprod Genet 2009; 26:597-604.
19. Asgari Z, Ghasemian F, Ramezani M, Bahadori MH. The effect of melatonin on the developmental potential and implantation rate of mouse embryos. Cell J 2012; 14:203-208.
20. Wang F, Tian X, Zhou Y, Tan D, Zhu S, Dai Y, et al. Melatonin improves the quality of in vitro produced (IVP) bovine embryos: implications for blastocyst development, cryotolerance, and modifications of relevant gene expression. PLoS One 2014; 9:e93641.
21. Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L, et al. The role of melatonin as an antioxidant in the follicle. J Ovarian Res 2012; 5:1.
22. Wang F, Tian X, Zhang L, Gao C, He C, Fu Y, et al. Beneficial effects of melatonin on in vitro bovine embryonic development are mediated by melatonin receptor 1. J Pineal Res 2014; 56:333-342.
23. Tian XZ, Wen Q, Shi JM, Liang W, Zeng SM, Tian JH, et al. Effects of melatonin on in vitro development of mouse two-cell embryos cultured in HTF medium. Endocr Res 2010; 35:17-23.
24. Choi J, Park SM, Lee E, Kim JH, Jeong YI, Lee JY, et al. Anti-apoptotic effect of melatonin on preimplantation development of porcine parthenogenetic embryos. Mol Reprod Dev 2008; 75:1127-1135.
25. He C, Wang J, Li Y, Zhu K, Xu Z, Song Y, et al. Melatonin‐related genes expressed in the mouse uterus during early gestation promote embryo implantation. J Pineal Res 2015; 58:300-309.
26. Chen YC, Sheen JM, Tiao MM, Tain YL, Huang LT. Roles of melatonin in fetal programming in compromised pregnancies. Int J Mol Sci 2013; 14:5380-5401.
27. Shih YF, Lee TH, Liu CH, Tsao HM, Huang CC, Lee MS. Effects of reactive oxygen species levels in prepared culture media on embryo development: a comparison of two media. Taiwan J Obstet Gynecol 2014; 53:504-508.
28. Reiter RJ, Tan D-X, Manchester LC, Paredes SD, Mayo JC, Sainz RM. Melatonin and reproduction revisited. Biol Reprod 2009; 81:445-456.
29. Zhao LH, Cui XZ, Yuan HJ, Liang B, Zheng LL, Liu YX, et al. Restraint stress inhibits mouse implantation: temporal window and the involvement of HB-EGF, estrogen and progesterone. PLoS One 2013; 8:e80472.
Iranian Journal of Basic Medical Sciences
Volume 20, Issue 6
June 2017
Pages 655-661

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