Evaluation of phytochemicals, antioxidant and burn wound healing activities of Cucurbita moschata Duchesne fruit peel

Document Type : Original Article

Authors

1 Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

3 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

4 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

5 Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran

6 Department of Drug and Food Control, Faculty of Pharmacy, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

7 Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

8 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

9 Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

10 Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

11 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Objective(s): Cucurbita moschata Duchesne (pumpkin) is a well-known plant with several pharmacological effects. The aim of the present study was to assess burn wound healing activity of C. moschata peel extract (CE). Also, standardized CE was assessed for antioxidant activity and antibacterial effects against major pathogens of burns.
Materials and Methods: Healing properties of topical preparation of 10% and 20% concentrations of CE were assessed on second degree burn in rats during a 14-day period as well as histological studies, total antioxidant power, lipid peroxidation and total thiol content of skin tissue samples.
Results: Radical scavenging IC50 and ferric-reducing antioxidant power value were 4.015±0.20 mg/ml and 142.63±2.65 mmol Fe2+/g, respectively. Total mucilage content was 13.8%. The optimal results were obtained by 20% CE that showed 90.80±5.86 % wound closure and tissue repair as well as significant reduction of tissue oxidative stress biomarkers. Histological analyses confirmed wound healing activity of pumpkin peel extract.
Conclusion: Considering the high mucilage content of the plant, providing a moist environment for wound, C. moschata peel extract could be a natural remedy for treatment of burns. Further clinical studies are suggested to confirm C. moschata peel extract as a wound healing agent.

Keywords

References

1.Bahramsoltani R, Farzaei MH, Rahimi R. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Arch Dermatol Res 2014; 306:601-617.
2.Wu F, Bian D, Xia Y, Gong Z, Tan Q, Chen J et al. Identification of major active ingredients responsible for burn wound healing of centella asiatica Herbs. Evid Based Complement Alternat Med 2012; 2012:848093.
3.Singer AJ, Clark RA. Cutaneous wound healing.  N Engl J Med 1999; 341:738-746.
4.Ching YH, Sutton TL, Pierpont YN, Robson MC, Payne WG. The use of growth factors and other humoral agents to accelerate and enhance burn wound healing. Eplasty 2011; 11:e41: 429-449.
5.Sahib AS, Al-Jawad FH, Alkaisy AA. Effect of antioxidants on the incidence of wound infection in burn patients. Ann Burns Fire Disasters 2010; 23:199-205.
6.Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. Ethnopharmacological approaches to wound healing-exploring medicinal plants of India. J Ethnopharmacol 2007; 114:103-113.
7.Rahimi R, Baghaei A, Baeeri M, Amin G, Shams-Ardekani MR, Khanavi M et al. Promising effect of Magliasa, a traditional Iranian formula, on experimental colitis on the basis of biochemical and cellular findings. World J Gastroenterol 2013; 19:1901-1911.
8.Farzaei MH, Ghasemi-Niri SF, Abdolghafari AH, Baeeri M, Khanavi M, Navaei-Nigjeh M et al. Biochemical and histopathological evidence on the beneficial effects of Tragopogon graminifolius in TNBS-induced colitis. Pharm Biol 2015; 53:429-436.
9.Caili F, Huan S, Quanhon L. A review on pharmacological activities and utilization technologies of pumpkin. Plant Foods Hum Nutr 2006; 61:73-80.
10.Wang SY, Huang WC, Liu CC, Wang MF, Ho CS, Huang WP, et al. Pumpkin (Cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice. Molecules 2012; 17:11864-11876.
11.Noor Aziah AA, Komathi CA. Physicochemical and functional properties of peeled and unpeeled pumpkin flour. J Food Sci 2009; 74:S328-333.
12.Kim MY, Kim EJ, Kim YN, Choi C, Lee BH. Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitacea) species and parts. Nutr Res Pract 2012; 6:21-27.
13.Ju LY, Chang D. Hypoglycemic effect of pumpkin powder. J Harbin Med 2001; 21:5-6.
14.Chen JG. Effects of sugar-removed pumpkin zymptic powders in preventing and treating the increase of blood glucose in alloxan-induced diabetic mice. Chin J Clin Rehabil 2005; 9: 94-95.
15.Jin H, Zhang YJ, Xiang JX,  Zhu LY, Chen P, Li J, Yao HY. Studies on the extraction of pumpkin components and their biological effects on blood glucose of diabetic mice. J Food Drug Anal 2013; 21:184-189.
16.Zhang ZJ. Effects of superfine pumpkin powder on alloxan induced Diabetes Mellitus rabbits. J Chin Cereals Oils Assoc 1998; 13:52–56.
17.Aghili, M, 1771. Makhzan-al-Advia. In: Rahimi R, Shams Ardekani MR, Farjadmand F.editors. Tehran: University of Medical Sciences Tehran;2009.
18.World Health Organization. Quality control methods for herbal materials. 2011.
19.Farzaei MH, Rahimi R, Attar F, Siavoshi F, Saniee P, Hadjimahmoodi M, et al. Chemical composition, antioxidant and antimicrobial activity of essential oil and extracts of Tragopogon graminifolius, a medicinal herb from Iran. Nat Prod Commun 2014; 9:121-124.
20.Farzaei MH, Khanavi M, Moghaddam G, Dolatshahi F, Rahimi R, Shams-Ardekani MR, et al. Standardization of Tragopogon graminifolius DC. extracts based on phenolic compounds and antioxidant activity. J Chem 2014; doi: 10.1155/2014/425965.
21.Mirmasumi M, Ebrahimzadeh H, Tabatabaei MF. Mucilage production in tissue culture of plantago lanceolata. J Agric Sci Technol 2001; 3:155-160.
22.Kalyansundaram NK, Amin DR, Dalal KC. Quality Evaluation of Isabgol Seeds. In: “Biannual Report (from Oct. 1978 to Nov. 1980) of All India Coordinated
Project on Medicinal and Aromatic Plants, Gujarat Agric. Unive., Anand: 1980.p.125-127.
23.Seghatoleslami S, Samadi N, Salehnia A, Azimi S. Antibacterial activity of endemic Satureja Khuzistanica Jamzad essential oil against oral pathogens. Iran Endod J 2009; 4:5-9.
24.Kahkeshani N, Farahanikia B, Mahdaviani P, Abdolghaffari A, Hassanzadeh G, Abdollahi M, et al. Antioxidant and burn healing potential of Galium odoratum extracts. Res Pharm Sci 2013; 8:197-203.
25.Navaei-Nigjeh M, Rahimifard M, Pourkhalili N, Nili-Ahmadabadi A, Pakzad M, Baeeri M, Abdollahi M. Multi-organ protective effects of cerium oxide nanoparticle/selenium in diabetic rats: evidence for more efficiency of nanocerium in comparison to metal form of cerium.  Asian J Anim Vet Adv 2012; 7:605-612.
26.Abdolghaffari AH, Baghaei A, Moayer F, Esmaily H, Baeeri M, Monsef-Esfahani HR, et al. On the benefit of Teucrium in murine colitis through improvement of toxic inflammatory mediators. Hum Exp Toxicol 2010; 29:287-295.
27.Sethi J, Sood S, Seth S, Talwar A. Evaluation of hypoglycemic and antioxidant effect of Ocimum sanctum. Indian J Clin Biochem  2004; 19:152-155.
28.Kovalik AC, Bisetto P, Pochapski MT, Campagnoli EB, Pilatti GL, Santos FA. Effects of an orabase formulation with ethanolic extract of Malva sylvestris L. in oral wound healing in rats. J Med Food 2014; 17:618-624.
29.Tamri P, Hemmati A, Boroujerdnia MG. Wound healing properties of quince seed mucilage: in vivo evaluation in rabbit full-thickness wound model. Int J Surg 2014; 12:843-847.
30.Farzaei MH, Abbasabadi Z, Shams-Ardekani MR, Abdollahi M, Rahimi R. A comprehensive review of plants and their active constituents with wound healing activity in traditional Iranian medicine. Wounds 2014; 26:197-206. 
31.Farzaei MH, Rahimi R, Abdollahi M, Abbasabadi Z. An evidence-based review on medicinal plants used for the treatment of peptic ulcer in traditional Iranian medicine. Int J Pharmacol 2013; 9:108-124.
32.Rahimi R, Mozaffari S, Abdollahi M. On the use of herbal medicines in management of inflammatory bowel diseases: a systematic review of animal and human studies. Dig Dis Sci 2009; 54:471-480.
33.Chonoko U, Rufai A. Phytochemical screening and antibacterial activity of cucurbita pepo (Pumpkin) against Staphylococcus aureus and Salmonella typhi.  Bayero J Pure App Sci 2011; 4:145-147.
34.Dang C. Effect of pumpkin distillable subject on lipid peroxidation and the activity of antioxidative enzyme induced by Plumbum in mouse. J Clin Rehabil 2004; 8:4378-4379.
35.Xia HC, Li F, Li Z, Zhang ZC. Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells. Cell Res 2003; 13:369-374.    
36.Yadav M, Jain S, Tomar R, Prasad GB, Yadav H. Medicinal and biological potential of pumpkin: an updated review. Nutr Res Rev 2010; 23:184-190.
37.Azizah AH, Wee KC, Azizah O, Azizah M. Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschato). Int Food Res J 2009; 16:45-51.
38.Pakrokh Ghavi P. The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots. Int J Biol Macromol 2015 75:51-57.
39.Prosky L, Asp NG, Furda I, DeVries JW, Schweizer TF, Harland BF. Determination of total dietary fiber in food and food products: collaborative study. J Assoc Off Anal Chem 1985; 68:677–679.
40.Broussard KC, Powers JG. Wound dressings: selecting the most appropriate type. Am J Clin Dermatol 2013; 14:449-459.
Iranian Journal of Basic Medical Sciences
Volume 20, Issue 7
July 2017
Pages 798-805

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