Histopathological study of erythropoietin protective effect on carbon monoxide-induced cardiotoxicity in rat

Document Type : Original Article


1 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University

2 Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University

3 Pharmaciutical Research Center, Institute of Neuropharmacology, Kerman University

4 Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Ahl Al Bayt, Karbala, Iraq

5 Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

6 Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

7 Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

8 Immunobiochemistry Laboratory, Immunology Research Center, Bu-Ali research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

9 Department of Pathology, School of Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

10 Electron Microscope Unit, Bu-Ali Research Institute, Mashhad University of Medical Science, Mashhad, Iran

11 School of Medicine, Mashhad Branch, Islamic Azad University, Mashhad, Iran


Objective(s): Cardiotoxicity is one of the major consequences in carbon monoxide poisoning. Following our previous work, in this study we aimed to define the myocardium changes induced by carbon monoxide (CO) intoxication and evaluate erythropoietin (EPO) effect on CO cardiotoxicity in rat.
Materials and Methods:  Severe carbon monoxide toxicity induced by 3000 ppm CO in Wistar rat.  EPO was administrated (5000 IU/Kg, intraperitoneal injection) at the end of CO exposure and then the animals were re-oxygenated with the ambient air. Subsequently heart was removed and assessed by histopathology and electron microscopy examinations.
Results: 3000 ppm CO induced significant myocardium injury; multiple foci of necrosis and lymphocyte infiltration compare with the control (P˂0.05). Electron microscopy examination showed myofibril lysis and mitochondrial swelling in myocardium due to 3000 ppm CO poisoning.  However EPO administration after CO exposure resulted in significant reduction in cardiomyocytes injury (P˂0.05).
Conclusion: Our results represented protective effect of EPO on cardiac injury induced by CO intoxication in rat.


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