The effect of eight weeks endurance training and high-fat diet on appetite-regulating hormones in rat plasma

Document Type : Original Article


1 Department of Human Science, Semnan University, Semnan, Iran

2 Exercise Physiology Research Canter, Baqiyatallah University of Medical Sciences, Tehran, Iran

3 Department of Exercise physiology, Faculty of Physical Education and Sports Science, University of Tehran, Tehran, Iran

4 Department of Biostatistics, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

5 Sport Science and Physical Education Research Center, Mashhad, Iran

6 Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Objective(s):Consumption of high-fat foods is one of the major causes of obesity. Physical exercise is a strategy used to counteract obesity. The aim of this study was to investigate the effect of eight weeks endurance training and high-fat diet (HFD) on appetite-regulating hormones in rat plasma.
Materials and Methods:Twenty eight male Wistar rats were randomly divided into four groups: Control group with standard diet (CSD), endurance training with a standard diet (ESD), control group with high-fat diet (CHFD) and endurance training with high-fat diet (EHFD). Twenty-four hr after the last training session, the blood samples were obtained and analyzed for hormones levels.
Results: The significant increased weight gain and food intake and decreased plasma nesfatin-1 and PYY3-36 levels were observed in CHFD group, while exercise under the HFD antagonized these effects. There were no significant changes in ghrelin, insulin and leptin levels in different groups.
Conclusion: These results suggest that exercise can prevent fattening effect of HFD. Probably, performing exercise makes a reduction of food intake and weight gain in rat via the increase in nesfatin-1 and PYY levels. However, further studies are necessary to understand the exact mechanisms involved in this field.


1. Bi S, Scott KA, Hyun J, Ladenheim EE, Moran TH. Running wheel activity prevents hyperphagia and obesity in Otsuka long-evans Tokushima Fatty rats: role of hypothalamic signaling. Endocrinology 2005; 146:1676-1685.
2. Ebal E, Cavalie H, Michaux O, Lac G. Effect of a moderate exercise on the regulatory hormones of food intake in rats. Appetite 2007; 49:521-524.
3. Elj NE, Lac G, Tabka Z, Gharbi N, Fezaa SE. Effect of physical exercise on reducing food intake and weight gain. Procedia Soc Behav Sci 2011; 30:2027-2031.
4. Bilski J, Teleglow A, Zahradnik-Bilska J, Dembinski A, Warzecha Z. Effects of exercise on appetite and food intake regulation. Med Sport 2009; 13:82-94.
5. Broom DR, Stensel DJ, Bishop NC, Burns SF, Miyashita M. Exercise-induced suppression of acylated ghrelin in humans. J Appl Physiol 2007; 102:2165-2171.
6. Stensel D. Exercise, appetite and appetite-regulating hormones: implications for food intake and weight control. Ann Nutr Metab 2010; 57:36-42.
7. Li J-B, Asakawa A, Li Y, Cheng K, Inui A. Effects of exercise on the levels of peptide YY and ghrelin. Exp Clin Endocrinol Diabetes 2011; 119:163-166.
8. Pałasz A, Krzystanek M, Worthington J, Czajkowska B, Kostro K, Wiaderkiewicz R, et al. Nesfatin-1, a unique regulatory neuropeptide of the brain. Neuropeptides 2012; 46:105-112.
9. Shimizu H, Oh S, Hashimoto K, Nakata M, Yamamoto S, Yoshida N, et al.Peripheral administration of nesfatin-1 reduces food intake in mice: the leptin-independent mechanism. Endocrinology 2009; 150:662-671.
10. Ghanbari-Niaki A, Kraemer RR, Soltani R. Plasma nesfatin-1 and glucoregulatory hormone responses to two different anaerobic exercise sessions. Eur J Appl Physiol 2010; 110:863-868.
11. Jurimae J, Maestu J, Jurimae T, Mangus B, von Duvillard SP. Peripheral signals of energy homeostasis as possible markers of training stress in athletes: a review. Metabolism 2011; 60:335-350.
12. Larson-Meyer DE, Palm S, Bansal A, Austin KJ, Hart AM, Alexander BM. Influence of running and walking on hormonal regulators of appetite in women. J Obes 2012; 2012:730409.
13. Leidy HJ, Dougherty KA, Frye BR, Duke KM, Williams NI. Twenty‐four‐hour ghrelin is elevated after calorie restriction and exercise training innon‐obese women. Obesity (Silver Spring) 2007; 15:446-455.
14. Mackelvie KJ, Meneilly GS, Elahi D, Wong AC, Barr SI, Chanoine J-P. Regulation of appetite in lean and obese adolescents after exercise: role of acylated and desacyl ghrelin. J Clin Endocrinol Metab 2007; 92:648-654.
15. Chelikani PK, Haver AC, Reidelberger RD. Comparison of the inhibitory effects of PYY (3-36) and PYY (1-36) on gastric emptying in rats. Am J Physiol Regul Integr Comp Physiol 2004; 287:R1064-R1070.
16. Ueda S-y, Yoshikawa T, Katsura Y, Usui T, Nakao H, Fujimoto S. Changes in gut hormone levels and negative energy balance during aerobic exercise in obese young males. J Endocrinol 2009; 201:151-159.
17. Ueda S-y, Yoshikawa T, Katsura Y, Usui T, Fujimoto S. Comparable effects of moderate intensity exercise on changes in anorectic gut hormone levels and energy intake to high intensity exercise. J Endocrinol 2009; 203:357-364.
18. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutr Res Rev 2010; 23:270-299.
19. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499-502.
20. MacLean PS, Higgins JA, Wyatt HR, Melanson EL, Johnson GC, Jackman MR, et al. Regular exercise attenuates the metabolic drive to regain weight after long-term weight loss. Am J Physiol Regul Integr Comp Physiol 2009; 297:R793-R802.
21. Chaolu H, Asakawa A, Ushikai M, Li Y-X, Cheng K-C, Li J-B, et al. Effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels in mice. Exp Ther Med 2011; 2:369-373.
22. Kohno D, Nakata M, Maejima Y, Shimizu H, Sedbazar U, Yoshida N, et al. Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding. Endocrinology 2008; 149:1295-1301.
23. Su Y, Zhang J, Tang Y, Bi F, Liu J-N. The novel function of nesfatin-1: anti-hyperglycemia. Biochem Biophys Res Commun 2010; 391:1039-1042.
24. Li Q-C, Wang H-Y, Chen X, Guan H-Z, Jiang Z-Y. Fasting plasma levels of nesfatin-1 in patients with type 1 and type 2 diabetes mellitus and the nutrient-related fluctuation of nesfatin-1 level in normal humans. Regul Pept 2010; 159:72-77.
25. Martins C, Morgan L, Truby H. A review of the effects of exercise on appetite regulation: an obesity perspective. Int J Obes (Lond) 2008; 32:1337-1347.
26. Ramezankhany A, Nazar AP, Hedayati M. Comparing effects of aerobics, pilates exercises and low calorie diet on leptin levels and lipid profiles in sedentary women. Iran J Basic Med Sci 2011; 14:256-263.
27. Broom DR, Batterham RL, King JA, Stensel DJ. Influence of resistance and aerobic exercise on hunger, circulating levels of acylated ghrelin, and peptide YY in healthy males. Am J Physiol Regul Integr Comp Physiol 2009; 296:R29-R35.
28. King JA, Wasse LK, Stensel DJ. The acute effects of swimming on appetite, food intake, and plasma acylated ghrelin. J Obes 2010; 2011:351628.
29. Christ ER, Zehnder M, Boesch C, Trepp R, Mullis PE, Diem P, et al. The effect of increased lipid intake on hormonal responses during aerobic exercise in endurance-trained men. Eur J Endocrinol 2006; 154:397-403.
30. Russell M, Misra M. Influence of ghrelin and adipocytokines on bone mineral density in adolescent female athletes with amenorrhea and eumenorrheic athletes. Med Sport Sci 2010; 55:103-113.
31. Erdmann J, Tahbaz R, Lippl F, Wagenpfeil S, Schusdziarra V. Plasma ghrelin levels during exercise effects of intensity and duration. Regul Pept 2007; 143:127-135.
32. Ghahraman M, Rohani H, AG. The acute effects of aerobic and resistance exercise on plasma acylated ghrelin and hungerin overweight men. World Appl Sci J 2013; 21:888-893.
33. King JA, Wasse LK, Broom DR, Stensel DJ. Influence of brisk walking on appetite, energy intake, and plasma acylated ghrelin. Med Sci Sports Exerc 2010; 42:485-492.
34. Ebrahimi M, Rahmani-Nia F, Damirchi A, Mirzaie B, Pur SA. Effect of short-term exercise on appetite, energy intake and energy-regulating hormones. Iran J Basic Med Sci 2013; 16:829-834.
35. Dimitriou L, Sharp N, Doherty M. Circadian effects on the acute responses of salivary cortisol and IgA in well trained swimmers. Br J Sports Med 2002; 36:260-264.
36. Hejazi K, Hosseini S-RA. Influence of selected exercise on serum immunoglobulin, testosterone and cortisol in semi-endurance elite runners. Asian J Sports Med 2012; 3:185-192.
37. McGuigan MR, Egan AD, Foster C. Salivary cortisol responses and perceived exertion during high intensity and low intensity bouts of resistance exercise. J Sports Sci Med 2004; 3:8-15.
38. Hazar S, Hazar M, Korkmaz S, Bayil S, Gurkan A. The effect of graded maximal aerobic exercise on some metabolic hormones, muscle damage and some metabolic end products in sportsmen. Sci Res Essays 2011; 6:1337-1343.
39. Fischer CP. Interleukin-6 in acute exercise and training: what is the biological relevance. Exerc Immunol Rev 2006; 12:6-33.
 40. Conn CA, Kozak WE, Tooten P, Niewold TA, Borer KT, Kluger MJ. Effect of exercise and food restriction on selected markers of the acute phase response in hamsters. J Appl Physiol 1995; 78:458-465.
41. Minetto M, Rainoldi A, Gazzoni M, Ganzit G, Saba L, Paccotti P. Interleukin-6 response to isokinetic exercise in elite athletes: relationships to adrenocortical function and to mechanical and myoelectric fatigue. Eur J Appl Physiol 2006; 98:373-382.
42. Stewart LK, Flynn MG, Campbell WW, Craig BA, Robinson JP, Timmerman KL, et al. The influence of exercise training on inflammatory cytokines and C-reactive protein. Med Sci Sports Exerc 2007; 39:1714-1719.
43. Ramson R, Jurimae J, Jurimae T, Maestu J. The influence of increased training volume on cytokines and ghrelin concentration in college level male rowers. Eur J Appl Physiol 2008; 104:839-846.
44. Yang N, Wang C, Xu M, Mao L, Liu L, Sun X. Interaction of dietary composition and PYY gene expression in diet-induced obesity in rats. J Huazhong Univ Sci Technolog Med Sci 2005; 25:243-246.
45. Sloth B, Holst JJ, Flint A, Gregersen NT, Astrup A. Effects of PYY1–36 and PYY3–36 on appetite, energy intake, energy expenditure, glucose and fat metabolism in obese and lean subjects. Am J Physiol Endocrinol Metab 2007; 292:E1062-E8.
46. Cooper J, Watras A, Paton C, Wegner F, Adams A, Schoeller D. Impact of exercise and dietary fatty acid composition from a high-fat diet on markers of hunger and satiety. Appetite 2011; 56:171-178.
47. Crouse SF, O’Brien BC, Grandjean PW, Lowe RC, Rohack JJ, Green JS. Effects of training and a single session of exercise on lipids and apolipoproteins in hypercholesterolemic men. J Appl Physiol 1997; 83:2019-2028.
48. Lee M-G, Park K-S, Kim D-U, Choi S-M, Kim H-J. Effects of high-intensity exercise training on body composition, abdominal fat loss, and cardiorespiratory fitness in middle-aged Korean females. Appl Physiol Nutr Metab 2012; 37:1019-1027.
49. Nounou HA, Deif MM, Shalaby MA. Effect of flaxseed supplementation and exercise training on lipid profile, oxidative stress and inflammation in rats with myocardial ischemia. Lipids Health Dis 2012; 11:129.
50. Greene NP, Fluckey JD, Lambert BS, Greene ES, Riechman SE, Crouse SF. Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women. Am J Physiol Endocrinol Metab 2012; 303:E1212-E1221.
51. Huffman KM, Hawk VH, Henes ST, Ocampo CI, Orenduff MC, Slentz CA, et al. Exercise effects on lipids in persons with varying dietary patterns-does diet matter if they exercise? Responses in Studies of a Targeted Risk Reduction Intervention through Defined Exercise I. Am Heart J 2012; 164:117-124.