Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats


1 Department of Biology, Faculty of Mathematic and Natural Sciences, Islamic University of Malang, Malang, East Java, Indonesia

2 Department of Pharmacology, Faculty of Medicine University of Brawijaya, Malang, East Java, Indonesia

3 Department of Cardiology and Vascular Medicine, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia


Objective(s):To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats.
Materials and Methods: The rats were divided in 5 groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All 5 groups received methanolic extract of S. atropurpurea (MESA) at a dosage of 50; 100; and 200 mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry.
Results: The administration of MESA100 and MESA200 elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group.  MESA100 and MESA200 also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< 0.05) decreased the CECs number compared to hypertensive groups.
Conclusion: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs.


1.Zhou W, Longhurst JC. Neuroendocrine mechanisms of acupuncture in the treatment of hypertension. Evid Based Complement Altern Med 2012; 2012.
2.McMGrath JC, Deighan C, Briones AM, Shafaroudi MM, McBride M, Adler J, et al. New aspects of vascular remodelling: the involvement of all vascular cell types. Exp Physiol 2005; 90:469–475.
3.Kondo S, Ueno H, Hashimoto J, Morizane C, Koizumi F, Okusaka T, et al. Circulating endothelial cells and other angiogenesis factor in pancreatic carcinoma patients receiving gemcitabine chemotherapy. BMC Cancer 2012; 12:268.
 4.Yu H, Shao H, Yan J, Tsoukias NM, Zhou MS. Bone marrow transplantation improves endothelial dunction in hypertensive Dahl salt-sensitive rats. J Am Soc Hypertens 2012; 6:331-337.
5.Badyal DK, Lata H, Dadhich AP. Animal models of hypertension and effect of drugs. Indian J Pharmacol 2003; 35:349-362.
6.Athiroh N, Permatasari N, Sargowo D, Widodo MA. Antioxidative and blood pressure-lowering effects of Scurrula atropurpurea on deoxycorticosterone acetate salt hypertensive rats. Biomarkers and Genom Med 2014; 6:32-36.
7.Smarason AK, Allman KG, Young D, Redman CW. Elevated levels of serum nitrate, a stable end product of nitric oxide, in women with pre-eclampsia. British J Obstet Gynaecol 1997; 104:538–543.
8.Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18:499–502.
9.Guo X, Liu L, Zhang M, Bergeron A, Cui Z, Dong JF,             et al. Correlation of CD34+ cells with tissue angiogenesis after traumatic brain injury in a rat model. J Neurotrauma 2009; 26:1337-1344.
10.Schmitt CA, Dirsch VM. Modulation of endothelial nitric oxide by plant-derived products. Nitric Oxide 2009; 21:77-91.
11.Mikirova NA, Casciari JJ, Hunninghake RE, Beezley MM. Effect of weight reduction on cardiovacular risk factors and CD34-positive cells in circulation. Int J Med Sci 2011; 8:445-452.
12.Iijima H, Daikonya A, Takamatsu S, Kanno A, Magariyama K, Yoshikawa K, et al. Effects of the herbal medicine composition “Saiko-ka-ryukotsu-borei-To” on the function of endothelial progenitor cells in hypertensive rats. Phytomedicine 2013; 20:196-201.
13.Cao Z, Yang Y, Hua X, Wu R, Wang J, Zhiu M, et al. Telmisartan promotes proliferation and differentiation of endothelial progenitor cells via activation of Akt. Chin Med J 2014; 127:109-113.
14.Liu JF, Du ZD, Chen Z, Han ZC, He ZX. Granulocyte colony-stimulating factor attenuates monocrotaline pulmonary hypertension by upregulating endothelial progenitor cells via nitric oxide system. Exp Ther Med 2013; 6:1402-1408.
15.Qi Y, Jiang Q, Chen C, Ca Y, Qian L. Circulating endothelial progenitor cells decrease in infants with bronchopulmonary dysplasia and increase after inhlaed nitric oxide. PLoS One 2013; 8.