Effect of Aqueous-Ethanolic Extract from Rosa damascena on Guinea Pig Isolated Heart

Document Type : Original Article


Department of Physiology and Pharmaceutical Research Centre, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran


In the present study, the effects of aqueous-ethanolic extract from Rosa damascena on heart rate and contractility were examined.
Materials and Methods
Isolated guinea-pig hearts were perfused through aorta in a Langendorff mode. Heart rate (HR) and contractility were determined in the presence of four concentrations of the extract (0.1, 0.2, 0.4 and 1.0 mg %) and isoprenaline (1, 10, 100 nM and 1 μM) in comparison with baseline values in the presence and absence of propranolol (n= 10 for each group).
Both isoprenaline and the extract caused increase in heart rate and contractility (P< 0.05 to P< 0.001). The percent increased in HR due to the final concentration of isoprenaline in the absence of propranolol was significantly greater than that of the extract (P< 0.01). Propranolol caused significant reduction in both HR and contractility (P< 0.05 for both) but this effect was significantly reversed by isoprenaline and the extract (P< 0.05 to P< 0.001). The percent increased in heart contractility due to the final concentration of the extract in the absence and presence of propranolol was significantly greater than that of isoprenaline (P< 0.05 for both cases). There was significant correlation between both HR and heart contractility with concentration of isoprenaline and the extract (P< 0.05 to P< 0.001).
In conclusion this study showed a relatively potent inotropic and chornotropic effect for Rosa damascena on isolated guinea-pig heart.


1. Libster M. Delmars integrative herb guide for nurses. Delmar 1999; 360-370.
2. Green M. The Rose.?? Aromatic thymes 1999; 7: 11-15.
3. Buckle J.Clinical aromatherapy in nursing. London: Arnold, co published by singular; 1997.
4. Wood G, Bache F.The dispensatory of the United States of America.4th ed. Philadelphia: Griggand Elliot;1833.
5. Ave-Sina. Law in Medicine, Interpreter; Sharafkhandy A. Tehran: Ministry of Guidance publication;1990.
6. Zargari A. Medicinal plants.5th ed. Tehran: Tehran University publications;1992.
7. Mahmood N, Piacenet S, Pizza C, Bruke A, Khan A, Hay A.The anti -HIV activity and mechanisms of action of pure compounds isolated from Rosa damascena. Biochem Biophys Res Commun 1996; 229:73-79.
8. Maleev A, Neshtev G, Stoianov S, Sheikov N.The ulcer protective and antiinflamatory effect of Bulgarian Rose oil. Eksp Med Morfol 1972; 11:55-60.
9. Hosseini M, Rakhshandah H, Shafieenic R, Dolati K. Analgesic effect of Rosa damascena on mice. Abstract book of 16th Iranian congress of physiology and pharmacology.2003. p.167.
10. Shafei MN, Boskabady MH. Antitussive effect of Rosa damascena in guinea pigs. Iran J Pharm Res 2003; 2:231-234.
11. Boskabady MH, Kiani S, Rakhshandeh H. Relaxant effects of Rosa damascena on guinea pig tracheal chains. J Ethnopharmacol 2006; 106:377-382.
12. Kwon EK, Lee DY, Lee H, Kim DO, Baek NI, Kim YE, et al. Flavonoids from the buds of Rosa damascene inhibit the activity of 3-Hydroxy-3-methylglutaryl-coenzyme a reductase and angiotensin I-converting enzyme. J Agric Food Chem 2010; 58: 882–886.
13. Boskabady MH, Ramazani M, Tabei T. Relaxant effects of different fractions of essential oil from carum copticum on guinea pig tracheal chains. Phytother Res 2003; 17:1145-1149.
14. Chlopicki S, Kozlovski VI, Gryglewski RJ. No-dependent vasodilation induced by nebivolol in coronary circulation is not mediated by beta-adrenoceptors or by 5 HT1A-receptors. J Physiol Pharmacol 2002; 53:615-624.
15. Chlopicki S, Kozlovski VI, Geygewski RJ. Clonidine-induced coronary vasodilation in isolated guinea pig heart is not mediated by endothelial a2 adrenoceptors. J Physiol Pharmacol 2003; 54:511-521.
16. Gilani AH, Janbaz KH, Aziz N, Herzig JU, Kazemi M, Choudhary MI, et al. Possible mechanism of selective inotropic activity of the n-butanolic fraction from Berberis aristata fruit. Gen Pharmacol 1999; 33:407-444.
17. Boskabady MH, Shafei MN, Parsaee H. Effects of aqueous and macerated extracts from Nigella sativa on guinea pig isolated heart. Pharmazie 2005; 60:943-948.
18. Boskabady MH, Shafei MN, Shakeeba A, Sangsefidi H. Effect of Crocus satirus on guinea pig isolated heart. Phytother Res 2005; 10:1002/ptr.2317
19. Sys SU, Boels PJ, Brutsaert DL. Positive inotropic and vasodilating effects of amrinone and milrinone in isolated canine heart. J Cardiovasc Pharmacol 1987; 10:445-449.
20. England PJ, Shahid M. Effects of forskolin on contractile response and protein phosphorylation in the isolated perfused rat heart. Biochem J 1987; 246: 687–695.
21. Lang RM, Fellner SK, Neumann A, Bushinsky DA, Borow KM. Left ventricular contractility varies directly with blood-ionized calcium. Ann Intern Med 1988; 108: 524–529.
22. Francis GS, Cohn J. Heart failure: mechanisms of cardiac and vascular dysfunction and the rationale for pharmacologic intervention. FASEB J 1990; 4:3068–3075.