Anti-inflammatory, Antipruritic and Mast Cell Stabilizing Activity of Aristolochia Indica

Document Type : Original Article

Authors

1 Department of Pharmaceutical Chemistry, MCOPS, Manipal University, Karnataka, India

2 Department of Oral Pathology, Yenepoya University, Mangalore, Karnataka, India

Abstract

Objective(s)
Aristolochia indica has been widely used in the traditional medicine for the treatment of a variety of diseases. In the present study different extracts of roots of A. indica were evaluated for their anti‌inflammatory, antipruritic and mast cell stabilizing activity.
Materials and Methods
Anti-inflammatory activity was performed by compound 48/80 induced rat paw edema model and antipruritic activity by examining the incidence of scratching behavior. Mast cell stabilizing activity was performed by compound 48/80 and sheep serum induced mast cell degranulation methods.
Results
The ethanol extract (300 mg/kg) and petroleum ether extract (100 mg/kg) were found to inhibit mast cell degranulation significantly equivalent to that of standard drug ketotifen (69%) by compound 48/80 model. In sheep serum model the ethanol extracts (150 and 300 mg/kg) and petroleum ether extract (100 mg/kg) showed good mast cell stabilizing activity (66-67%). Ethanol extract at 150 mg/kg showed 70% reduction of rat paw oedema and also significantly reduced the scratching response.
Conclusion
Results suggest A. indica has good mast cell stabilizing, anti-inflammatory and antipruritic activity.

Keywords

References

1.The Wealth of India. A dictionary of Indian Raw Material and Industrial Products. CSIR, New Delhi, India: 1950.
2.Henry TA. The Plant Alkaloids. J & A Churchill, London: 1949.
3.Pakrashi A, Shaha C. Effect of methyl ester of aristolic acid from Aristolochia indica Linn. on fertility of female mice. Experientia 1978; 34:1192-1193.
4.Nagasawa H, Wu G, Inatomi H. Effects of aristoloside, a component of Guan-mutong (Caulis aristolochiae manshuriensis), on normal and pre-neoplastic mammary gland growth in mice. Anticancer Res 1997; 17:237-240.
5.Prachi G, Haruyo I, Nikita M, Gautam S, Bharat BA. From ancient medicine to modern medicine: ayurvedic concepts of health and their role in inflammation and cancer. J Soc Integr Oncol 2007; 5:25-37.
6.Achari B, Chakrabarty S, Pakrashi SC. Studies on Indian medicinal plants: An N-glycoside and steroids from Aristolochia indica. Phytochemistry 1981; 20:1444-1445.
7.http://www.planetayurveda.com/natural-herbs-atoz/a-natural-herbs/aristolochia-indica.htm
8.Bhandari MJ, Chandrashekhar KR, Kaveriappa KM. Medical ethnobotany of the Siddis of Uttara Kannada district, Karnataka, India. J Ethnopharmacol 1995, 47:149-158.
9.Bhandari MJ, Chandrashekhar KR, Kaveriappa KM. Ethnobotany of Gowlis of Uttara Kannada district, Karnataka. J Economic Taxonomical Botany, 1996; 12:244-249.
10.Ghosh MN. Fundamentals of experimental pharmacology. Calcutta: Scientific Book Agency; 1984.
11.Mastuda H, Yasuko IDO, Hirata A, Yoshiaki INO. Antipruritic effect of Cnidii monnieri fructus. Biol Pharm Bull 1997; 25:260-263.
12.Ishiguro K, Oku H, Kato T. Antipruritic effects of 1,4-Naphthoquinones and related compounds. Biol Pharm Bull 2002; 25:137-139.
13.Shin TY. Inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by the aqueous extract of Mentha arvensis. Immunopharmacol Immunotoxicol 2003; 25:273-283.
14.Loeffler LJ, Lovenberg W, Sjoerdsma A. Effects of dibutyryl-1-3,5-cyclic adenosine monophosphate, phosphodiestarases inhibitors and prostaglandin E1 on compound 48/80 induced histamine release from rat peritoneal mast cells in vitro. Biochem Pharmacol 1971; 20:2278-2297.
15.Lee YM, Kim SH, Kim DK, Shin TY, Kin HM. Antianaphylactic activity of Poncirus trifoliate fruit extract. J Ethanopharmacol 1996; 54:77-84.
16.Noguchi K, Matsuo K, Ohata N. Synthesis of peptide related to immunoglobulin E (IgE) and the examination of their pharmacological activity. Chem Pharm Bull 1990; 38:2463-2466.
17.Prussin C, Metcalfe DD. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 2003; 111:486¬494.
18.Mousli MC, Bronner C, Bockaert J, Rouot B, Landry Y. Interaction of substance P, compound 48/80 and mastoparan with a-subunit C-terminal of G protein. Immunol Lett 1990; 25:355-358.
Iranian Journal of Basic Medical Sciences
Volume 14, Issue 5 - Serial Number 5
September 2011
Pages 422-427

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