Improvement effect of Lycium barbarum polysaccharide on sub-health mice

Document Type : Original Article


1 Department of Pharmaceutical Engineering, College of Life Science & Biotechnology, Heilongjiang August First Land Reclamation University, Daqing High-Tech Industrial Development Zone, 163319, P.R. China

2 Department of Pharmaceutical Engineering, College of Life Science & Biotechnology, Heilongjiang Bayi Agricultural University, Daqing High-Tech Industrial Development Zone, 163319, P.R. China

3 Department of physical education, Hebei Normal University of Science and Technology, 360 Hebei Street, Qinhuangdao 066004, P.R. China


Objective(s):Sub-health has been described as a chronic condition of unexplained deteriorated physiological function, which falls between health and illness. In the present study, we evaluated the effects of Lycium barbarum polysaccharide (LBP), a polysaccharide fraction purified from Lycium barbarum (L. barbarum) on the sub-health mice.
Materials and Methods:The sub-health model mice were built through compound factors. The mice were given intragastric administration of LBP at low dose (50 mg•kg-1) and high dose (100 mg•kg-1), respectively. After LBP treatment for 4 weeks, the antioxidant ability, enhancing immune function and anti-fatigue activity were detected.
Results:The results showed that LBP could enhance antioxidant ability in sub-health mice. LBP could effectively improve immunity of sub-health mice and protect the immune organs, such as thymus. In addition, LBP showed anti-fatigue ability in sub-health mice.
Conclusion:LBP could improve sub-health state caused from composite factor through three aspects, such as increasing antioxidant ability, promoting T lymphocyte proliferation, inhibiting thymus lymphocyte apoptosis, and alleviating fatigue.


1. Dunstan RH, Sparkes DL, Roberts TK, Crompton MJ, Gottfries J, Dascombe BJ. Development of a complex amino acid supplement, Fatigue Reviva™, for oral ingestion: initial evaluations of product concept and impact on symptoms of sub-health in a group of males. Nutr J 2013; 12:115-123.
2. Wang W, Russell A, Yan Y, Global Health Epide-miology Reference Group (GHERG). Traditional Chinese medicine and new concepts of predictive, preventive and personalized medicine in diagnosis and treatment of suboptimal health. EPMA J 2014; 5:4-13.
3. Huyan T, Li Q, Yang H, Jin ML, Zhang MJ, Ye LJ, et al. Protective effect of polysaccharides on simulated microgravity-induced functional inhibition of human NK cells. Carbohydr Polym 2014;101:819-827.
4. Chen JR, Li EQ, Dai CQ, Yu B, Wu XL, Huang CR, et al. The inducible effect of LBP on maturation of dendritic cells and the related immune signaling pathways in hepatocellular carcinoma (HCC). Curr Drug Deliv 2012; 4:414-420.
5. Zhao R, Li QW, Li J, Zhang T. Protective effect of Lycium barbarum polysaccharide 4 on kidneys in streptozotocin induced diabetic rats. Can J Physiol Pharmacol 2009;87: 711-719.
6. Singh A, Naidu PS, Gupta S, Kulkarni SK. Effect of natural and synthetic antioxidants in a mouse model of chronic fatigue syndrome. J Med Food 2002; 5:211-220.
7. Rajeevan MS, Dimulescu I, Murray J, Falkenberg VR, Unger ER. Pathway-focused genetic evaluation of immune and inflammation related genes with chronicfatigue syndrome. Hum Immunol 2015; 15:00180-9.
8. Zhao R, Qiu B, Li Q, Zhang T, Zhao H, Chen Z, et al. LBP-4a improves insulin resistance via translocation and activation of GLUT4 in OLETF rats. Food Funct 2014; 5:811-820.
9. Wang J, Sun CX, Zheng Y, Pan H, Zhou Y, Fan Y. The effective mechanism of the polysaccharides from Panax ginsengon chronic fatigue syndrome. Arch Pharm Res 2014; 37:530-538.
10. Li YP, Huang SH, Jin W. Construction of a sub-health fatigue rat model. Chin J Tissue Engin Res 2011; 7:1225-1228.
11. Jiang CM, Chen XL, Lu GF, Chen XY, Wang DY, Hu YL, et al. Comparative study on chicken peripheral lymphocyte and spleen lymphocyte proliferation by Chinese herbal medicinal polysaccharides in vitro. Jiangsu J of Agr Sci  2015; 31:106-111.
12. Luo Q, Cai Y, Yan J, Sun M, Corke H. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sci2004; 76:137-149.
13. Tian DH. Practical thesaurus of Chinese traditional medicine. People’s Medical Publishing House, Beijing; 2002.p.1348-1354.
14. Wang J. Pharmacological action of Lycium barbarum and the analysis of clinical application value. Asia-Pacific Traditional Med2014; 10:50-51.
15. Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 2008; 88:1243-1276.
16. Ekert PG, Vaux DL. The mitochondrial death squad: hardened killers or innocent bystanders? Curr Opin Cell Biol 2005; 6:626-630.
17. Joza N, Susin SA, Daugas E, Stanford WL, Cho SK, Li CYJ. Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 2001; 6828: 549-554.
18. Susin SA, Daugas E, Ravagnan L, Samejima K, Zamzami N, Loeffler M, et al. Two distinct pathways leading to nuclear apoptosis. J Exp Med 2000; 4:571-580.
19. Li J, Jin YJ. Epidemiological surveys of sub-health state and syndrome differentiation of Traditional Chinese Medicine. Chin J Clin Rehabil 2004; 9:1756.
20. Castro-Marrero J,  Cordero MD, Sáez-Francas N, Jimenez-Gutierrez C, Aguilar-Montilla FJ, Aliste L, et al. Could mitochondrial dysfunction be a differentiating marker between chronic fatigue syndrome and fibromyalgia? Antioxid Redox Signal 2013; 15:1855-60.
21. Filler K, Lyon D, Bennett J,  McCain N, Elswick R, Lukkahatai N, et al. Association of mitochondrial dysfunction and fatigue: a review of the literature. BBA Clin 2014; 1:12-23.