Prednison provokes serum and vasoactive substances in a mice model of immune thrombocytopenia

Document Type : Original Article

Authors

1 Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China

2 School of Pharmacy, Xi’an Medical College, Shanxi 710021, China

3 No. 1 Hospital Affiliated to Guiyang College of TCM, GuiYang 550001, China

4 No. 1 Hospital Affiliated to Guangzhou University of Chinese Medicine, Guangzhou 510405, China

Abstract

Objective(s): The main objective of this study was to investigate the variations of β-endorphin (β-EP), vasoactive intestinal peptide (VIP), serotonin (5-HT) and norepinephrine (NE) of immune thrombocytopenia (ITP) mice as well as the regulatory mechanism of prednison.
Materials and Methods: Sixty BALB/c mice were randomly divided into control group, model group and prednison intervention group. ITP mice model was duplicated by injecting with glycoprotein-antiplatelet serum (GP-APS) except in control group. After ITP disease model was successful established, prednison was used in prednison intervention group. The β-EP, VIP, 5-HT and NE contents of ITP mice were detected by enzyme linked immunosorbent assay (ELISA).
Results:Compared with the values in control group, the detection values of VIP and 5-HT in model group declined, while the detection values of β-EP and NE increased. Compared with prednison intervention group, the detection values of VIP and 5-HT in model group increased, while the detection values of β-EP and NE showed no significant change.
Conclusion: In this study, the β-EP, VIP, 5-HT and NE contents in ITP mice injected with GP-APS were changed by prednison. It shows that prednison as the first-line therapy for ITP with effective hemostasis function is likely to increasing the contents of VIP and 5-HT. These results suggest the therapeutic value of prednison for the treatment of ITP.

Keywords

References

1. Singh A, Solanki A. High dose methylprednisolone: effective in pregnancy associated post-splenectomy refractory chronic immune thrombocytopenic purpura [J]. NJOG 2014; 2:64-66.
2. Pang MX, Huang XL, Li QY. Clinical observation of the combined applications of High-dose intravenous immunoglobulin and dexamethasone to treating idiopathic thrombocytopenic purpura of children. Acta Med Sinica 2012; 25:154-157.
3. Lang HY, Ma W, Zhang YY, SHI FQ, CHEN XY. Effect of long dan sheng xue granule on the peripheral hemogram, bone marrow megakaryocytes and serum PAIgG level in model mice with thrombocytopenic purpura. J Med Res 2014; 43:91-94.
4. Feng TT, Liu YB, Li ZY, et al. Production of monoclonal antibodies against enrofloxacin and the establishment of enzyme-linked immunosorbent assay. Isotopes, 2009; 22:5.
5. Provan D, Stasi R, Newland AC, Blanchette VS, Bolton-Maggs P, Bussel JB, et al. International consensus report on the investigation and management of primary immune thrombocytopenia. Blood 2010; 115:168-186.
6. Chen L, Liu XL. Relationship between β-endorphin and the therapeutic effect of opioids on the treatment of cancerous pain. Pain Clin J 2011; 7:63-66.
7. Zhu WL, Li JD, Zhang LF, Li H. Influence of moxibustion on content of β-endorphin in rats with adjuvant arthritis. J Beijing Univ Traditional Chinese Med 2011; 18:1-3.
8. Zhao WL, Zhao WS, Huang HW, Zhong LL. Effects of electroacupuncture on the hypothalamicβ-endorphin in rats with bone cancer pain. J Clin Acupuncture Moxibustion, 2013; 29:69-72.
9. Zang YS, Ji XL. Relationship between Anxiety and Serum β-endorphin in patients with Vitiligo. J Shanghai Jiaotong Univ 2012; 32:1347-1350.
10. Cheng H, Li RJ, Liang XL, et al. β-endorphin level expression in severe burn patients with depression in period of rehabilitation. J Med Theory Pract 2013; 26:985-990.
11. Huang C, Han LM. Electroacupuncture and immunity. Shandong Med J 2012; 52:91-92.
12. Cheng XW, Zheng QH, Li XL, et al. The research progress of pathogenic relationship between vasoactive intestinal peptide and some gastric motility disorders. Chin Gen Pract 2012; 15:237-240.
13. Jiang M, Liu DC. Vasoactive intestinal peptide and cardiovascular activity. Chongqing Med 2011; 40:1026-1028.
14. Ding F, Yang Z, Li G. Research progress of vasoactive intestinal peptide in COPD-related pulmonary hypertension. Int J Respir 2014; 34:1667-1670.
15. Gao RM, Lin X, Meng XY, Wang Q. Expression of motilin and vasoactive intestinal peptide in gastric mucosa of patients with primary bile reflux gastritis. World Chin J Digestol 2010; 18:722-725.
16. Zhou HY, Jiang HW, Wang MJ. Research progress of serotonin(5-HT) and its receptor. Shandong Med 2014; 54:90-93.
17. Chen M, Wang HF, Wang Y, Zhang XM. Meta-analysis of comparative efficacy between dopamine and norepinephrine in the treatment of septic shock. Shandong Med 2015; 55:52-54.
18. Liu C, Hu Z. Research progress of dopamine and norepinephrine for the treatment of septic shock. Med Recapitulate, 2011; 17:1361-1363.
Iranian Journal of Basic Medical Sciences
Volume 19, Issue 9
September 2016
Pages 1010-1015

Files

Share

How to cite

Statistics